D termination de la destin e cellulaire des neurones de la r tine de vert br s
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Détermination de la destinée cellulaire des neurones de la rétine de vertébrés. Muriel Perron Janvier 2005 Master 2 Module Neuro-Evo-Devo. Développement de l’œil de vertébrés. La rétine: excellent modèle d ’étude pour la biologie neuro-développementale. accessibilité

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Détermination de la destinée cellulaire des neurones de la rétine de vertébrés

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D termination de la destin e cellulaire des neurones de la r tine de vert br s

Détermination de la destinée cellulaire des neurones de la rétine de vertébrés

Muriel Perron

Janvier 2005

Master 2

Module Neuro-Evo-Devo


D termination de la destin e cellulaire des neurones de la r tine de vert br s

Développement de l’œil de vertébrés


La r tine excellent mod le d tude pour la biologie neuro d veloppementale

La rétine: excellent modèle d ’étude pour la biologie neuro-développementale

  • accessibilité

  • nombre limité de neurones

  • organisés en couches


Les cellules r tiniennes

Les cellules rétiniennes

  • Photoreceptors, rods and cones, are found in the outer layer of the retina. Their outer segments are membranous structures that capture light and carry out phototransduction.

  • They form synapses with bipolar and horizontal cells, found in the inner nuclear layer.

  • Also in the inner nuclear layer are amacrine cells, which synapse with bipolar cells and the output cell type, ganglion cells.

  • Ganglion cells then send the result of all of this processing to the brain via the optic nerve.


Neuronal genesis in the retina

Neuronal genesis in the retina

Klassen et al., 2004


Pluripotent retinal progenitors

Pluripotent retinal progenitors

  • Clonal analysis:the daughters of a single progenitor injected with horseradish peroxidase contribute many different cell types.

Holt et al., 1988


What is the importance of the lineage

What is the importance of the lineage?

Mu and Klein, 2004


Cell lineage analysis

Cell lineage analysis

  • Fluorescent dextran was injected into single cells of the embryonic optic vesicle.

    • Labeled descendants were observed in all three layers of the larval retina.

    • Furthermore, different clones were composed of various combinations of all major cell types, including the glial Muller cells.

    • Hence, single optic vesicle cells have the potential to form any type of retinal cell, suggesting that the interactions that specify the differentiation pathway of retinal cells must occur late in development.

Wetts R, Fraser SE. 1987


Cell lineage analysis1

Cell lineage analysis

  • Retrovirus-mediated gene transfer was used to mark cell lineages in vivo in the postnatal rat retina.

    • Labelled clones contained up to three different cell types: three types of neurons or two types of neurons and a Muller glial cell.

    • This indicates that a single retinal progenitor can generate remarkably diverse cell types near the end of development.

Turner and Cepko 1987


Lineage independent determination of cell type in the embryonic mouse retina

Lineage-independent determination of cell type in the embryonic mouse retina

Model for the generation of retinal cell types in which the cessation of mitosis and cell type determination are independent events.

Turner and Cepko, 1987

Turner et al., 1990

Wetts and Fraser, 1988

Holt et al., 1988


Models for the cell fate choice

Models for the cell fate choice


Are extrinsic or intrinsic cues important for cell fate determination

Are extrinsic or intrinsic cues important for cell fate determination?


Extrinsic factors

Extrinsic factors

  • Testing retinal cells for cell fate choices in different environments, e.g. after adding factors to cultures


Growth factors

Growth factors

Fibroblast growth factor-2

  • Addition of FGF-2 to cultured optic vesicles causes presumptive pigmented epithelium to undergo neuronal differentiation whereas neutralizing antibodies to FGF-2 block neural differentiation in the presumptive retina. FGF-2 also accelerates the appearance of differentiated ganglion cells in retinal explants.

    Transforming growth factor-alpha

  • In vitro, low concentrations of TGF-alpha stimulate proliferation, whereas high concentrations inhibit rod differentiation and promote Müller cell differentiation.

    Transforming growth factor-ß

  • Transforming growth factor-ß stimulates production of retinal amacrine cells while photoreceptor production remains unchanged.

Pittack et al., 1997; Zhao et al., 1996; Lillien, 1995; Anchan et al., 1995; Harris, 1997


Hormonal factors

Hormonal factors

Retinoic acid

  • Application of RA to zebrafish causes precocious differentiation of rods in postmitotic cells. When the synthesis of endogenous RA is inhibited, rod differentiation is impeded. RA treatment of dissociated rat cell cultures specifically increases the number of progenitors that develop as photoreceptors and decreases the number of cells that develop as amacrine cells.

    Thyroid hormone

  • TH induces an increase in the number of cone photoreceptors in embryonic rat retinal cultures.

Hyatt et al., 1996 ;Kelley et al, 1994 ; Stenkamp et al., 1993 ; Kelley et al., 1995


Neurotrophic factors

Neurotrophic factors

Ciliary neurotrophic factor

  • Addition of CNTF to postnatal rat retinal explants results in a dramatic decrease in rod differentiation and an increase in bipolar differentiation, suggesting that postmitotic cells which would normally differcntiate into rods switch their fate and differentiate as bipolar cells in response to CNTF; consistent with this, more cells differentiate as rods in mouse retinal explants lacking a functional CNTF receptor.

Ezzeddine et al. 1997


Neurotrophic factors1

Neurotrophic factors

  • CNTF can drive cells fated to be rods to express features of the bipolar neuron phenotype and fail to express rod markers. In this case, although the cells are specified to become rods, an extrinsic signal can change the fate of these cells.

Ezzeddine et al. 1997


Feedback inhibition

Feedback inhibition

  • There are soluble factors produced by postmitotic neurons that provide feedback inhibition to progenitors to regulate cell-fate choices

Belliveau et Cepko, 1999


Placing cells in new cellular environments heterochronic transplant experiments

Placing cells in new cellular environments: heterochronic transplant experiments

  • progenitors from different stages of development were placed in an environment of a different age (either earlier or later).

  • early retinal progenitors, when cocultured with cells from the late stage of histogenesis, failed to give rise to late-born retinal cells

    • Morrow et al., 1998 Belliveau and Cepko, 1999 Rapaport et al., 2001

  • conversely, late retinal progenitors failed to generate early-born retinal neurons when cultured with cells from the late stage of histogenesis

    • Morrow et al., 1998


Changes in competence progenitors over time

Changes in competence progenitors over time


The competence model

The competence model

All these findings led to the development of the COMPETENCE model of retinal development, which proposed that progenitors pass through a series of competence states, during each of which the progenitors are competent to produce a subset of retinal cell types.

Livesey and Cepko2001


Cell cell interactions

Cell-cell interactions


Notch delta signaling pathway

Notch/Delta signaling pathway

Jun Hatakeyama, Ryoichiro Kageyama 2004


The development of photoreceptor polarity in the eye antennal imaginal disc of drosophila

The development of photoreceptor polarity in the eye-antennal imaginal disc of Drosophila

Blair 1999


Changes in photoreceptor specification induced by the loss or gain of notch activity

Changes in photoreceptor specification induced by the loss or gain of Notch activity

Blair 1999


D termination de la destin e cellulaire des neurones de la r tine de vert br s

C20

Rôle de la cascade Notch dans le choix de la destiné des précurseurs rétiniens chez les vertébrés?


D termination de la destin e cellulaire des neurones de la r tine de vert br s

Technique de micro-injection chez le xénope

DNA à étudier

Analyse du phénotype

Stade 2-32 cellules


Delta misexpression in the retina

Delta misexpression in the retina

f. Control.

g. When the blastomere is injected with Delta (green), almost all the retinal descendants are in the ganglion cell layer and the photoreceptor layer. Almost all the Delta+ photoreceptors are double labeled with a cone marker (inset).

Dorsky et al., 1997


D termination de la destin e cellulaire des neurones de la r tine de vert br s

Delta misexpression in the retina

  • Delta-misexpressing cells adopt earlier fates, primarily becoming ganglion cells and cone photoreceptors.

Injection au

stade 16 cellules

Dorsky et al., 1997


D termination de la destin e cellulaire des neurones de la r tine de vert br s

Lipofection in vivo

DNA

+

GFP DNA

+

Dotap


D termination de la destin e cellulaire des neurones de la r tine de vert br s

Lipofection in vivo avec la GFP


Delta misexpression in the retina1

Delta misexpression in the retina

  • Delta-misexpressing cells adopt earlier fates, primarily becoming ganglion cells and cone photoreceptors.

  • Progenitors transfected with Delta later in development also produce rod photoreceptors.

  • importance of timing in Delta function.

Injection au

stade 16 cellules

Lipofection

au stade neurula

Dorsky et al., 1997


Model to generate cellular diversity through notch delta signaling

Model to generate cellular diversity through Notch/Delta signaling

Delta signaling in the vertebrate retina is a basic regulatory mechanism that can be used to generate neuronal diversity.

Perron and Harris, 1999


Intrinsic factors

Intrinsic factors

  • Identifying and testing transcription factors that might play a role in retinal cell fate decision


Les facteurs bhlh

Les facteurs bHLH

Bertrand et al., 2002


Vertebrate proneural genes fall into two groups ash and ath genes

Vertebrate proneural genes fall into two groups: Ash and Ath genes

  • The Ash group (Ash1, Ash2 and Ash3) is composed of bHLH proteins with homology to Drosophila AS-C complex genes.

  • The Ath genes have well-conserved bHLH amino acid sequences homologous to another Drosophila proneural gene, atonal. These include the Ath, Ngn and NeuroD subfamilies.

Reviewed in Vetter and Brown, 2001


Bhlh factors in the nervous system

bHLH factors in the nervous system

In the nervous system, bHLH factors have been proposed to coordinate the acquisition of both general neuronal properties and subtype-specific features of differentiated neurons.


Bhlh factors in the nervous system1

bHLH factors in the nervous system

Different neural bHLH proteins, expressed in distinct dorsoventral progenitor domains of the spinal cord, control the specification of different interneuron subtypes


Role of bhlh factors in retinal cell fate determination

Role of bHLH factors in retinal cell fate determination?


D termination de la destin e cellulaire des neurones de la r tine de vert br s

ZMC = zone marginale ciliaire

Epithélium

pigmenté

Cellules souches

Rétine neurale

Neuroblastes

Cristallin

Précurseurs en

différenciation

ZMC

Nerf

optique


Expression of bhlh gene xath5

Expression of bHLH gene Xath5

  • Xath5 s’exprime dans les précurseurs rétiniens, mais ni dans les cellules souches, ni dans les cellules différenciées

Kanekar et al., 1997


D termination de la destin e cellulaire des neurones de la r tine de vert br s

Overexpression of bHLH gene Xath5

*

50

GFP

45

Xath5

40

35

30

25

20

*

15

10

*

5

0

Ganglionnaire

Amacrine

Bipolaire

Horizontale

Photorécepteur

Müller

La surexpression de Xath5 conduit à une augmentation des cellules ganglionnaires et une diminution des cellules de Müller et bipolaires


Loss of function of bhlh gene xath5

Loss of function of bHLH gene Xath5

  • Absence of retinal ganglion cells in lak mutants

Kay et al., 2001


Role of bhlh factors in retinal cell fate determination1

Role of bHLH factors in retinal cell fate determination

  • RGCs require Ath5

  • amacrine cells and photoreceptors require NeuroD

  • bipolar cells require Ash1 and Ath3

  • photoreceptor cells and bipolar cells require Ngn2

Hatakeyama et Kageyama, 2004

Reviewed in Vetter and Brown, 2001


Bhlh repressor factors

bHLH repressor factors

  • The Hes/Her class of antagonistic genes is named for their sequence and functional homology with Drosophila hairy and E(spl) genes. These proteins inhibit neurogenesis through direct transcriptional repression of proneural genes.


D termination de la destin e cellulaire des neurones de la r tine de vert br s

WRPW

helix loop helix

basique

orange

HC

Groucho

LA PROTEINE HES


D termination de la destin e cellulaire des neurones de la r tine de vert br s

EXPRESSIONDE HES

vésicule

otique

oeil

HES a une expression très restreinte au cours du développement


D termination de la destin e cellulaire des neurones de la r tine de vert br s

st. 30

st. 35

st. 40

EXPRESSIONDE HES AU COURS DE LA RETINOGENESE


D termination de la destin e cellulaire des neurones de la r tine de vert br s

*

*

*

*

*

*

*

*

*

*

*

*

*

*

La surexpression de Hes conduit à une augmentation des cellules gliales de Müller ganglionnaires et une inhibition de la neurogenèse

Control

1244 cells

11 retinas

Hes

939 cells

13 retinas

% of retinal cells

80

60

40

20

*

0

Ganglionnaire

Amacrine

Bipolaire

Horizontale

Photorécepteur

Müller


D termination de la destin e cellulaire des neurones de la r tine de vert br s

XHes2-∆WRPW (dominant négatif)

XHes2-∆WRPW-VP16 (antimorphe)

domaine d’activation

de VP16

LES CONSTRUCTIONS DE HES

XHes2

Groucho

WRPW

helix loop helix

basique

orange

HC


D termination de la destin e cellulaire des neurones de la r tine de vert br s

HES

GLIOGENESE

NEUROGENESE

Generation of Müller glia by bHLH repressors

80

Control

70

60

XHes2

∆WRPW

4

VP16

2

Cellules de Müller


Role of other transcription factors in retinal cell fate determination

Role of other transcription factors in retinal cell fate determination?


D termination de la destin e cellulaire des neurones de la r tine de vert br s

Prox1, an homeodomain transcription factor, is both necessary and sufficient for the formation of horizontal cells

Dyer et al., 2003; Cook 2003


D termination de la destin e cellulaire des neurones de la r tine de vert br s

Requirement of the bHLH genes Mash1/Math3 and thehomeodomain gene Chx10 for bipolar cell fate specification

Misexpression of Mash1 or Math3 alone predominantly generates photoreceptors.

Misexpression of Chx10 alone generates INL cells, but they are Müller glia or undifferentiated cells.

(c) In contrast, misexpression of Mash1 or Math3 with Chx10 generates many bipolar cells.

Hatakeyama et Kageyama 2004


Cooperation of bhlh and homeodomain genes for retinal cell type specification

Cooperation of bHLH and homeodomain genes for retinal cell type specification

Hatakeyama et Kageyama 2004


Role of the homeodomain transcription factor foxn4

Role of the homeodomain transcription factor Foxn4


Foxn4 is expressed solely by a subset of mitotic progenitors

Foxn4 is expressed solely by a subset of mitotic progenitors.

Liu et al., 2004


Defect in the genesis of amacrine and horizontal cells in foxn4 retinas

Defect in the Genesis of Amacrine and Horizontal Cells in Foxn4_/_ Retinas

Liu et al., 2004


Reduction of math3 neurod1 and prox1 expression in foxn4 retinas

Reduction of Math3, NeuroD1, and Prox1 Expression in Foxn4_/_ Retinas

Math3, NeuroD1, and Prox1, whichareretinogenic genes required for the generation of amacrine and horizontal cells, are reduced in Foxn4 -/- retinas.

Otherretinogenic genes are not affected in Foxn4 -/- retinas.


Overexpressed foxn4 promotes the formation of amacrine cells

Overexpressed Foxn4 Promotes the Formation of Amacrine Cells

  • What factor define the competence state of retinal progenitors ?

  • This transcription factor defines the amacrine-generating competence state.

Liu et al., 2004


D termination de la destin e cellulaire des neurones de la r tine de vert br s

ProposedMechanism by which Foxn4 Controls the Genesis of Amacrine and Horizontal Cells by Retinal Progenitors


Nouvelles approches

Nouvelles approches

  • Molecular differences among progenitor cells using the microarray technology


D termination de la destin e cellulaire des neurones de la r tine de vert br s

Résumé

Harris, 1997


D termination de la destin e cellulaire des neurones de la r tine de vert br s

There are several subtypes of each class of neurons…For example: twenty or more different types of amacrine cells


D termination de la destin e cellulaire des neurones de la r tine de vert br s

Differentiation of a particular subtype of cell

Control of late off-center cone bipolar cell differentiation by the homeobox gene Vsx1The transcription factor Bhlhb4 is required for rod bipolar cell maturation

Bramblett et al., 2004; Chow et al., 2004


D termination de la destin e cellulaire des neurones de la r tine de vert br s

Conservation des molécules impliquées dans la détermination des neurones rétiniens au cours de l’evolution?

Kumar 2001


D termination de la destin e cellulaire des neurones de la r tine de vert br s

‘It requires little persuasion to be convinced that the lens eye of a vertebrate and the compound eye of an insect are independent evolutionary events.’

Ernst Mayr

1961


D termination de la destin e cellulaire des neurones de la r tine de vert br s

Comparaisons de structures de rétines, de leur origine embryonnaire, position des axones…..

Les yeux auraient évolués indépendamment entre 40 et 65 fois au cours de l’évolution.

Salvini-Plawen and Mayr, 1961


D termination de la destin e cellulaire des neurones de la r tine de vert br s

Perte de fonction eyeless/Pax6

Surexpression de eyeless dans divers tissus

tête

aile

eyeless

Small eye

patte

antenne

Surexpression chez la drosophile du gène Pax6 de souris

1995

Walter Gehring


D termination de la destin e cellulaire des neurones de la r tine de vert br s

Conclusions

Les gènes eyeless de drosophile et Pax6 de vertébrés sont interchangeables

L’œil de drosophile et celui de vertébrés utilisent le même « gène maître ».

L’œil de drosophile et celui de vertébrés auraient une origine commune...


Qu en est il de la d termination des neurones r tiniens

Qu’en est-il de la détermination des neurones rétiniens?


R tinogen se chez les vert br s et la drosophile

Vertebrate

Drosophila

Rétinogenèse chez les vertébrés et la drosophile

As in the vertebrate eye, the commitment of each retinal cell type within the fly eye occurs from a pool of pluripotent retinal precursors in a sequential, stereotyped order.

Cook 2003


Conservation of pathways that regulate retinogenesis across evolution

Conservation of pathways that regulate retinogenesis across evolution

  • role for Prox1 transcription factor in specifying horizontal cells in the mouse retina

  • Prospero, the Drosophila homolog of Prox1, also participates in retinal cell specification


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