Selection-free screening protocol for plant transformation: an open-source platform for plant biotechnology The GusPlus ™ project. A new GUS gene, Available under BIOS TM licensing, Pioneering use of the BioForge TM concept. TM. GUSPlus TM as a selectable marker. Premise:
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A new GUS gene,
Available under BIOSTM licensing,
Pioneering use of the BioForgeTM concept
Based on our observations, plant tissues could survive and continue to regenerate after incubation in a low concentration X-GlcA solution, and potentially in the presence of the end product of GUS cleavage (indigo).
A number of approaches have been used:
Tobacco callus (upper left) or tobacco shoot (lower left) showing GUS expression (arrow) after incubation
with low concentration X-GlcA. These tissues regenerated into plantlets whose leaves also expressed GUS
(upper and lower right).
Summary of GUSPlus plantsTM selection for transgenic tobacco plants
Rice callus expressing GUS after incubation in low concentration X-GlcA.
GUS-expressing callus was cultured on regeneration media and some of the developing plantlets
expressed GUS in leaf material.
Summary of GUSPlus plantsTM selection for transgenic rice plants
Arabidopsis seedling screened using low concentration X-GlcA showing GUS expression in the roots (arrows)
Leaf from same plant after 2 weeks growth in soil, stained with X-glcA to show GUS expression.
Summary of GUSPlus plantsTM selection for transgenic Arabidopsis plants
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The requirements GUSPlus™ project
Funded by the Rockefeller Foundation, Monticello Research Foundation and Horticulture Australia
A new screening protocol for transgenic plants
Brian Weir, Heidi Mitchell, Tuan Nguyen,Richard Jefferson
Draft License: Mat Berman (UC) Mike Rabson, Marie Connett Porceddu, Richard Jefferson; Commentable website: Steve Irwin, Nick dos Remedios;
BioForgeTM distributive collaboration website
Collabnet® and CAMBIA’s BIOS Initiative