Lab safety
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LAB SAFETY. Apparel. Shoes Lab coats Safety glasses. Note!. No eating, drinking, or smoking in the lab at any time. Spills. Wash Notify Receive instructions. Accidents. Be careful! Pay attention Report any injuries. Other Matters. Broken glass Other waste Pipetting

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LAB SAFETY

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Lab safety

LAB SAFETY


Apparel

Apparel

  • Shoes

  • Lab coats

  • Safety glasses


Lab safety

Note!

No eating, drinking, or smoking in the lab at any time.


Spills

Spills

  • Wash

  • Notify

  • Receive instructions


Accidents

Accidents

  • Be careful!

  • Pay attention

  • Report any injuries


Other matters

Other Matters

  • Broken glass

  • Other waste

  • Pipetting

  • Volatile chemicals

  • Dirty labware

  • Labeling

  • General cleanliness


Aseptic technique

ASEPTIC TECHNIQUE


I contaminants

I. Contaminants

A. Bacteria, fungi, and insects

B. Initial contaminants

C. Latent or persistent contamination

D. Introduced contamination

E. Detection of contaminants


The transfer hood

THE TRANSFER HOOD

  • The hood should remain on

    • Necessary items present, unnecessary things removed

  • Check the bottom of the hood for blockage

  • Wash hands


The transfer hood1

THE TRANSFER HOOD

  • Spray or wipe the inside of the transfer hood

  • Wipe hands and lower arms with 70% EtOH

  • Remove dangling sleeves and jewelry


Sterilization and use of supplies and equipment

Sterilization and use of supplies and equipment

  • Know which of your implements, flasks, etc. are sterile and which are not

  • Monitor autoclave time

  • Sterilized items should be used within a short time

  • Items that come packaged sterile should be examined carefully for damage before use


Sterilizing tools vessels etc

Sterilizing tools, vessels etc.

  • Autoclaving

  • Ethylene oxide gas

  • UV radiation

  • Dry heat

  • Microwave


Working in the transfer hood

Working in the transfer hood:

  • Work well back in the transfer hood

  • Make sure that materials in use are to the side of your work area, so that airflow from the hood is not blocked

  • Instruments (scalpels, forceps) can be sterilized by flaming or immersion in hot beads


Working in the transfer hood1

Working in the transfer hood:

  • Plant material should be placed on a sterile surface when manipulating it in the hood

  • Remove items from the hood as soon as they are no longer needed

  • When transferring plant cultures, do contaminated cultures last.


Working in the transfer hood2

Working in the transfer hood:

  • When finished in the hood, clean up after yourself.

  • Be sure when you are finished that you turn off the gas to the burner

  • It is pointless to practice good sterile technique in a dirty lab


Surface sterilizing plant material

Surface-sterilizing plant material

  • Care of stock plants

  • Alcohol

  • Bleach

  • Calcium hypochlorite

  • Mercuric chloride

  • Hydrogen peroxide

  • Rinsing


In the media

In the media

  • Use of antibiotics and fungicides

  • Plant Preservative Mixture

  • Rinsing


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