Fabrication of Novel Biomaterials. for Peripheral Nerve Tissue. Engineering Strategies. Archit Sanghvi, David Silva, Kiley Miller, Julie Williams,. Angela Belcher, Christine Schmidt. Motivation.
(1) Physical modification of biomaterials
(2) Chemical modification of biomaterials
Y-shaped bifurcated conduit
100 mm optical polymer waveguide splitter; pattern representative of a bifurcated pathway
MediLabTM ink-jet based Tissue Engineering Platform
24 hr study illustrating cell viability using live/dead assay (green-live, red-dead)
72 hr study illustrating cell viability using live/dead assay (green-live, red-dead
Cells: PC12 (rat adrenal tumor cells) neuron-like cells used in study
Material: Copolymer of poly-lactic acid (PLA) and poly-ethylene glycol (PEG)
*Control: Cells were plated on the same surface area using plexiglass wells on polystyrene TC dishes
Conclusion: Results showed excellent potential for NGC development
using Microfab polymer, because in vitro studies demonstrated
comparable PC12 adhesion and viability
SEM of Microfab polymer (PLA-PEG) on glass slide, illustrating precise-control fabrication
Biotin moieties presented at the polymer surface are used to immobilize tetrameric avidin molecules. Free biotin binding sites on the avidin molecules are in turn used to anchor biotinylated ligands such as, bNGF.