Colors uv vis spectroscopy
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Colors  UV – VIS spectroscopy. Jakub Kovář  Jiří Stančík. Color and light. The starting point of an understanding of color is a description of light. White light is actually made up of a whole range of colours, mixed together We can see this if we pass white light through a glass prism.

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Colors  UV – VIS spectroscopy

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Colors uv vis spectroscopy

ColorsUV – VIS spectroscopy

Jakub Kovář  Jiří Stančík


Color and light

Color and light

  • The starting point of an understanding of color is a description of light.

  • White light is actually made up of a whole range of colours, mixed together

  • We can see this if we pass white light through a glass prism


Light spectrum

Light spectrum

  • The colors produced by light passing through a prism are arranged in a precise array or spectrum from red through orange, yellow, green, blue, indigo and into violet

  • The intensity of light as a function of wavelength is called light spectrum


Light spectrum1

Light spectrum

  • The visible spectrum of light is just a small part of the electromagnetic spectrum which extends from radio waves (long waves) to gamma rays (10-3 meters down to 10-15 meters, the size of the nucleus)


The color of objects

The color of objects

  • Color is produced by the absorption of selected wavelengths of light by an object

  • The receptors of the human eye only respond to light in the visible spectrum


Mixing of colors

Mixing of colors

  • RGB model uses red, green and blue color

  • CMYK model uses colors: cyan, magenta, yellow and black

  • Basic characteristics of colors are: hue, saturation, brightness


Uv vis spectroscopy

UV – VIS spectroscopy

  • Analytical method

  • Light is a form of electromagnetic radiation

  • This radiation contains radio waves, visible light and X-rays

  • Light rays are composed of photons whose energy specifies a color from red to violet

  • A visible spectrum has many colors, and a rainbow is a typical spectrum


Wavelenght of colors in uv vis spectrum

Wavelenght of colors in UV – VIS spectrum

  • Visible region: 380 – 700 nm

  • Violet: 400 nm

  • Blue: 480 nm

  • Green: 500

  • Yellow: 580 nm

  • Orange: 600 nm

  • Red: 700 nm


Scheme of spectrophotometer

Scheme of spectrophotometer


Description of spectrophotometer

Description of spectrophotometer

  • Source of radiation(tungsten lamp, laser …)

  • Monochrome (prism, graticule…)

  • Sample compartment(cuvette with sample)

  • Detector (diode-array detector)


Principles of spectroscopy

Principles of spectroscopy

  • Spectrometer exposes a chemical solution to the UV – VIS light across the electromagnetic spectrum from about 300 to 800 nm

  • A detector then measures the amount of light transmitted through the solution

  • The amount of light reached by the detector is then recorded as a spectrum


Theory

Theory

  • Since UV-VIS spectroscopy is an absorption technique, Lambert - Beer's law can be applied

  • Spectrophotometer


Vocabulary

Vocabulary

  • Prism - krystal

  • Hue - odstín

  • Saturation - sytost

  • Brightness – světlost

  • Rain bow – dešťová poklona 


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