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Colors  UV – VIS spectroscopy. Jakub Kovář  Jiří Stančík. Color and light. The starting point of an understanding of color is a description of light. White light is actually made up of a whole range of colours, mixed together We can see this if we pass white light through a glass prism.

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colors uv vis spectroscopy

ColorsUV – VIS spectroscopy

Jakub Kovář  Jiří Stančík

color and light
Color and light
  • The starting point of an understanding of color is a description of light.
  • White light is actually made up of a whole range of colours, mixed together
  • We can see this if we pass white light through a glass prism
light spectrum
Light spectrum
  • The colors produced by light passing through a prism are arranged in a precise array or spectrum from red through orange, yellow, green, blue, indigo and into violet
  • The intensity of light as a function of wavelength is called light spectrum
light spectrum1
Light spectrum
  • The visible spectrum of light is just a small part of the electromagnetic spectrum which extends from radio waves (long waves) to gamma rays (10-3 meters down to 10-15 meters, the size of the nucleus)
the color of objects
The color of objects
  • Color is produced by the absorption of selected wavelengths of light by an object
  • The receptors of the human eye only respond to light in the visible spectrum
mixing of colors
Mixing of colors
  • RGB model uses red, green and blue color
  • CMYK model uses colors: cyan, magenta, yellow and black
  • Basic characteristics of colors are: hue, saturation, brightness
uv vis spectroscopy
UV – VIS spectroscopy
  • Analytical method
  • Light is a form of electromagnetic radiation
  • This radiation contains radio waves, visible light and X-rays
  • Light rays are composed of photons whose energy specifies a color from red to violet
  • A visible spectrum has many colors, and a rainbow is a typical spectrum
wavelenght of colors in uv vis spectrum
Wavelenght of colors in UV – VIS spectrum
  • Visible region: 380 – 700 nm
  • Violet: 400 nm
  • Blue: 480 nm
  • Green: 500
  • Yellow: 580 nm
  • Orange: 600 nm
  • Red: 700 nm
description of spectrophotometer
Description of spectrophotometer
  • Source of radiation(tungsten lamp, laser …)
  • Monochrome (prism, graticule…)
  • Sample compartment(cuvette with sample)
  • Detector (diode-array detector)
principles of spectroscopy
Principles of spectroscopy
  • Spectrometer exposes a chemical solution to the UV – VIS light across the electromagnetic spectrum from about 300 to 800 nm
  • A detector then measures the amount of light transmitted through the solution
  • The amount of light reached by the detector is then recorded as a spectrum
theory
Theory
  • Since UV-VIS spectroscopy is an absorption technique, Lambert - Beer\'s law can be applied
  • Spectrophotometer
vocabulary
Vocabulary
  • Prism - krystal
  • Hue - odstín
  • Saturation - sytost
  • Brightness – světlost
  • Rain bow – dešťová poklona 
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