Fibre properties that affect paper quality. Strength Microfibril length/thickness Hydrogen bonding between microfibrils and other cell wall constituents Cell length Cell wall thickness Length Cell expansion (longitudinal vs. lateral) Cell division (rate and/or extent)
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30K cDNA 70mer oligomer cDNA arrays
What additional genes emerge as trait-associated?
What are relevant stages of development to investigate?
How can we compare expression profiles between ecotypes?
How do we relate the expression data to the QTL study?
What do these array experiments mean if no QTLs are identified?
How do we relate the Arabidopsis fibre story to poplar?
What interesting biological stories can be investigated in parallel
with the strategic grant objectives?
“…seems likely that biophysical properties of fibres are closely linked to certain biological processes, such as cambial cell cycling, cell elongation, cell wall synthesis and lignification.”
Ehlting et al., 2005
Larkin et al., 2003
What is the signaling story surrounding IFF development?
…relating to the establishment of fibre phenotypes?
Auxin JA GA
Local organization centres in root development
Extreme Ecotypes or RILs
Isolate mRNAs from IFFs and other cell types (FACs or LCM/RNA) at different developmental stages
Expression profiling of developmentally-equivalent tissues between ecotypes and RILs
Fibre QTL analysis (Phenotyping +Genotyping of RILs)