1 / 5

Evidence for the presence of XMRV in 33 PCR Negative US CFS Patients

Evidence for the presence of XMRV in 33 PCR Negative US CFS Patients . 19/33 Antibodies in the plasma 30/33 Transmissable virus in the plasma 10/33 Protein expression in Decitibine (5Aza2DC) treated PBMC

pules
Download Presentation

Evidence for the presence of XMRV in 33 PCR Negative US CFS Patients

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Evidence for the presence of XMRV in 33 PCR Negative US CFS Patients 19/33 Antibodies in the plasma 30/33 Transmissable virus in the plasma 10/33 Protein expression in Decitibine (5Aza2DC) treated PBMC Thus, since the submission to Science we determined 99 of 101 US patients show evidence of XMRV infection

  2. Since the submission to Science, we have +-replicated at the NCI/CC our findings in other cohorts not in the WPI repository. 9/15 (60%) positive for XMRV gag DNA from fresh PBMC 13/15 (86.7%) positive by western for XMRV Env and Gag upon co-culture of plasma or PBMC with LNCaP 8/15 (53%) plasma samples contain antibody to XMRV Env 9/15 (60%) positive for XMRV gag DNA from fresh PBMC 13/15 (86.7%) positive by western for XMRV Env and Gag upon co-culture of plasma or PBMC with LNCaP 8/15 (53%) plasma samples contain antibody to XMRV Env XMRV Expression in NC/FL Cohort Since the submission to Science, we have replicated at the NCI/CC our findings in other cohorts not in the WPI repository.

  3. Familial Transmission of XMRV From Plasma from patients with Childhood Alzheimer’s Acute flulike infection in the family Mother/Father XMRV Env/Antibody positive (no active virus detected) XMRV envelope protein in LNCaP from children’s plasma

  4. XMRV Associated Neuroimmune Diseases (XAND):Potential Candidates: Atypical MS: 3/3 positive for XMRV ENV protein and gag DNA Fibromyalgia: 12/20 (60%) positive for XMRV gag DNA Autism: 6/15 (40%) positive for XMRV gag DNA 4/7 ( 57%) positive for serum Antibody to XMRV Env Gulf War Illness : Not Tested Samples were taken from family members of XMRV positive CFS subjects with these neuroimmune diseases.

  5. ~ 77 of the Nevada cohort have a clonal TCRg rearrangement More than 100 of these patients have clonal populations of gamma delta T cells Gamma delta T cells play an active role in the regulation and resolution of pathogen induced immune responses. They accumulate at sites of inflammation associated with viral, bacterial and parasitic infections and in auto immune diseases Interestingly, Gamma Delta T cells up-regulate MIP1lalpha and Beta , TNF alpha, TCR g Clonality in Nevada CFS Cohort

More Related