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VaporPrint TM of Infectious Bacteria Cultures

Fresh Nutrient. Strep pneumonia. Shigella flexneri. Salmonella enteritidis. Salmonella typhimurium. Klebsiella pneumonia. Pseudomonas aeruginosa. Candida albicans. Staph aureus. Hemophilus influenza. Enterococcus faecalis. E. Coli. E. Coli O157.

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VaporPrint TM of Infectious Bacteria Cultures

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  1. Fresh Nutrient Strep pneumonia Shigella flexneri Salmonella enteritidis Salmonella typhimurium Klebsiella pneumonia Pseudomonas aeruginosa Candida albicans Staph aureus Hemophilus influenza Enterococcus faecalis E. Coli E. Coli O157 VaporPrintTM of Infectious Bacteria Cultures WWW.ESTCAL.COM Cultures Prepared by: Department of Pathology and Laboratory Medicine, State Public Health Laboratory of Nevada WWW.ESTCAL.COM

  2. An Investigation of Infectious Bacteria With a GC/SAW Electronic Nose • Water based cultures were inoculated with 12 infectious bacteria and allowed to incubate at 37oC for 24 hours before analyzing headspace vapors with GC/SAW Electronic nose. Two of the twelve cultures did not develop and contained no bacteria. • Headspace vapor of cultures without bacteria displayed two primary nutrient analyte peaks and three small analyte peaks located midway between the two nutrient peaks. • Bacteria growth dynamically interacted with nutrient compounds as well as created new analyte vapors specific to the bacteria itself. The concentrations of new analytes together with modified nutrient vapors created VaporPrintsTM images specific to bacteria type. • E.Coli and E.Coli )157 produced nearly identical VaporPrintsTM, however, one analyte was found to be specific to only O157. • In general each bacteria type could be recognized by its unique VaporPrintTM image. WWW.ESTCAL.COM

  3. Hemophilus influenza Nutrient Broth Strep pneumonia Nutrient Broth No Growth Confirmed for Hemophilus influenza and Strep pneumonia Culture Bottles WWW.ESTCAL.COM

  4. Candida albicans Candida albicans Nutrient Broth WWW.ESTCAL.COM

  5. Shigella flexneri Nutrient Broth Shigella flexneri WWW.ESTCAL.COM

  6. Staph aureus Nutrient Broth Staphylococcus aureus WWW.ESTCAL.COM

  7. Pseudomonas aeruginosa Nutrient Broth Pseudomonas aeruginosa WWW.ESTCAL.COM

  8. Salmonella enteritidis Nutrient Broth Salmonella enteritidis WWW.ESTCAL.COM

  9. Enterococcus faecalis Nutrient Broth Enterococcus faecalis WWW.ESTCAL.COM

  10. Klebsiella pneumonia Nutrient Broth Klebsiella pneumonia WWW.ESTCAL.COM

  11. E. coli O157 Nutrient Broth Escherichia coli O157 WWW.ESTCAL.COM

  12. E. Coli (benign) Nutrient Broth E. coli (benign) WWW.ESTCAL.COM

  13. Comparison of E. coli E. coli O157 E. coli (benign) Specific to O157 E. Coli WWW.ESTCAL.COM

  14. Salmonella typhimurium Nutrient Broth Salmonella typhimurium WWW.ESTCAL.COM

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