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Supported by grant from Brazilian Scientific Foundations: FAPESP (08/53969-0), CAPES, CNPq. - PowerPoint PPT Presentation


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Fabio Carvalho , Linda C Carrijo-Carvalho , Ana Marisa Chudzinski-Tavassi, Annette Foronda, Denise de Freitas . Low-molecular weight proteins as potential virulence factors of Acanthamoeba pathogens in corneal infection.

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Fabio Carvalho, Linda C Carrijo-Carvalho, Ana Marisa Chudzinski-Tavassi, Annette Foronda, Denise de Freitas

Low-molecular weight proteins as potential virulence factors of Acanthamoeba pathogensin corneal infection

Supported by grant from Brazilian Scientific Foundations: FAPESP (08/53969-0), CAPES, CNPq.


Background

Corneal infections due to free-living amoebae have potentially devastating consequences. If not successfully treated, it can progress into the eye, causing corneal ulcer, loss of visual acuity and eventually blindness.

BACKGROUND


Background1

Proteases represent a class of enzymes which are related with pathogenicity and cytolysis of Acanthamoeba species and genotypes.

BACKGROUND


Purpose

To evaluate the expression of extracellular proteins by with pathogenicity and cytolysis of Acanthamoeba trophozoites as predictive virulence factors in the corneal infection by the protozoa.

PURPOSE


Methods

METHODS


Methods1
METHODS of the Declaration of Helsinki


Results

KDa of the Declaration of Helsinki

109

95

46

36

RESULTS

Lane 1:

  • Negative control

    Lane 2:

  • ATCC 30011 avirulent strain

    Lanes 3 and 6:

  • Single pattern

    Lane 4:

  • High diversity


Results1

KDa of the Declaration of Helsinki

141

117

96

76

55

36

RESULTS

Lane 8:

  • Single pattern

    Lanes 11 and 12:

  • High diversity


  • AK: role in virulence.

Low molecular weight protein


Conclusions

CONCLUSIONS


Conclusions1

Collagenases should be suggested as different Acanthamoeba isolates; a virulence marker in the differentiation of Acanthamoeba species and genotypes.

CONCLUSIONS


Perspectives

PERSPECTIVES


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