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Fabio Carvalho , Linda C Carrijo-Carvalho , Ana Marisa Chudzinski-Tavassi, Annette Foronda, Denise de Freitas . Low-molecular weight proteins as potential virulence factors of Acanthamoeba pathogens in corneal infection.

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Supported by grant from Brazilian Scientific Foundations: FAPESP (08/53969-0), CAPES, CNPq.

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Supported by grant from brazilian scientific foundations fapesp 08 53969 0 capes cnpq

Fabio Carvalho, Linda C Carrijo-Carvalho, Ana Marisa Chudzinski-Tavassi, Annette Foronda, Denise de Freitas

Low-molecular weight proteins as potential virulence factors of Acanthamoeba pathogensin corneal infection

Supported by grant from Brazilian Scientific Foundations: FAPESP (08/53969-0), CAPES, CNPq.


Background

Corneal infections due to free-living amoebae have potentially devastating consequences. If not successfully treated, it can progress into the eye, causing corneal ulcer, loss of visual acuity and eventually blindness.

BACKGROUND


Background1

Proteases represent a class of enzymes which are related with pathogenicity and cytolysis of Acanthamoeba species and genotypes.

BACKGROUND


Purpose

To evaluate the expression of extracellular proteins by Acanthamoeba trophozoites as predictive virulence factors in the corneal infection by the protozoa.

PURPOSE


Methods

  • The research was conducted in accordance with the tenets of the Declaration of Helsinki

  • Corneal scrapings

    • 10 patients

    • Contact-lens wearers

  • Acanthamoeba keratitis isolates (n=10)

METHODS


Methods1

METHODS


Results

KDa

109

95

46

36

RESULTS

Lane 1:

  • Negative control

    Lane 2:

  • ATCC 30011 avirulent strain

    Lanes 3 and 6:

  • Single pattern

    Lane 4:

  • High diversity


Results1

KDa

141

117

96

76

55

36

RESULTS

Lane 8:

  • Single pattern

    Lanes 11 and 12:

  • High diversity


Supported by grant from brazilian scientific foundations fapesp 08 53969 0 capes cnpq

  • Collagenases

  • AK: role in virulence.

Low molecular weight protein


Conclusions

  • Heterogeneous extracellular collagenases are produced by different Acanthamoeba isolates;

  • Avirulent Acanthamoeba strain could not produce collagenolytic enzymes;

CONCLUSIONS


Conclusions1

Collagenases should be suggested as a virulence marker in the differentiation of Acanthamoeba species and genotypes.

CONCLUSIONS


Perspectives

  • The genotyping and phylogenetic inferences of clinical isolates are currently under investigation;

  • Collagenolytic enzymes are under isolation by chromatographic techniques.

PERSPECTIVES


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