Fig s1
This presentation is the property of its rightful owner.
Sponsored Links
1 / 4

Fig. S1 PowerPoint PPT Presentation


  • 37 Views
  • Uploaded on
  • Presentation posted in: General

HincII. HincII. XbaI. XbaI. Wild type allele. Probe. Probe. Targeting vector. Neo. XbaI. XbaI. Targeted allele. Neo. p53. +/+. +/+. +/-. +/-. -/-. -/-. mkk7. mkk7. MDM2. +/-. +/-. -/-. -/-. p-ERK. +/-. +/-. -/-. -/-. p-p38. +/-. +/-. -/-. -/-.

Download Presentation

Fig. S1

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript


Fig s1

HincII

HincII

XbaI

XbaI

Wild type allele

Probe

Probe

Targeting vector

Neo

XbaI

XbaI

Targeted allele

Neo

p53

+/+

+/+

+/-

+/-

-/-

-/-

mkk7

mkk7

MDM2

+/-

+/-

-/-

-/-

p-ERK

+/-

+/-

-/-

-/-

p-p38

+/-

+/-

-/-

-/-

Whole embryos x 5

Liver x 200

+/-

+/-

-/-

-/-

a

Fig. S1

b

c

Kbp

mkk7

neo allele

15

wild type allele

12

actin

d

Penninger


Fig s2

Fig. S2

mkk7 +/-

mkk7 -/-

0 h

0 h

H2O2, 48h

H2O2, 48h

G1: 59%

S: 10%

G2/M 31%

G1: 67%

S: 14%

G2/M 19%

G1: 70%

S: 12%

G2/M 18%

G1: 55%

S: 7.8%

G2/M 37%

mkk7 +/-

mkk7 -/-

40

30

The full scan for Fig. 2a

G2/M (%)

20

TNFa

UV

10

-/-

mkk7

+/+

+/-

+/+

+/-

-/-

+/+

+/-

-/-

mkk7

+/-

-/-

-/-

0

mkk4

-

-

+

0

48

0

48

GST-c-Jun

86

(h) after H2O2

69

pBabe

mkk7

(kDa)

52

mkk7-/-

mkk7+/+

mkk7

+/+

+/-

-/-

+/+

+/-

-/-

MKK4

121

40

86

184

control

control

control

control

pBabe

mkk7

mkk7

121

69

26

52

86

121

69

JNK

86

40

(kDa)

69

52

184

26

MKK7

52

40

121

40

86

26

69

26

(kDa)

(kDa)

52

a-actin

MKK7

The full scan for Fig. 2c

JNK

40

The full scan for Fig. 3b

26

The full scan for Fig. 2d

a

b

c

d

e

f

Penninger


Fig s3

Fig. S3

mkk7+/-

mkk7-/-

1

4

7

10

1

4

7

10

121

mkk7

+/+

+/-

-/-

86

184

61

121

49

mkk7

+/+

+/-

-/-

86

36

Cdc2

69

184

32

121

52

MKK4

121

86

86

69

61

40

mkk7

+/+

+/-

-/-

+/+

+/-

-/-

mkk7

49

p53

52

CyclinD1

184

36

184

121

184

32

40

121

86

184

121

86

61

36

121

ATF-2

69

86

32

86

26

52

49

c-Jun

61

69

p21

20

40

36

Jun D

15

52

49

JNK

32

40

+/-

-/-

mkk7

+/-

-/-

+/-

-/-

+/-

26

-/-

121

121

p16

20

mkk7

86

86

+/+

+/-

-/-

mkk7

+/+

+/-

-/-

184

15

69

86

61

61

The full scan for Fig. 4b

121

GST-

c-Jun

184

61

86

121

49

49

HH1

Cdc2

CycB1

52

49

p-c-Jun

86

61

26

36

61

40

26

49

49

MAPK

Jun B

36

28

26

mkk7+/-

mkk7-/-

Passage No.

3

6

8

3

6

8

mkk7

+/+

+/-

-/-

121

cdc2

mkk7

mkk7

mkk7

mkk7

+/+

+/-

-/-

+/+

+/+

+/+

+/-

+/-

+/-

-/-

-/-

-/-

86

61

49

p38

26

28S

mkk7

+/+

+/-

-/-

18S

121

86

61

49

36

Cdc2

32

a

The full scan for Fig. 4a

b

c

d

The full scan for Fig. 4c

JNK

The full scan for Fig. 4d

Penninger


Fig s4

Fig. S4

mkk7

-/-

-/-

+/-

+/-

mkk7

2

5

mkk7-/-

Passages

c-Jun

AA

-/-

AA

-/-

+/+

+/+

Control

121

c-Jun

86

+/-

-/-

+/-

-/-

actin

61

49

49

cdc2

36

36

Cdc2

hgf

Cdc2

met

32

32

61

2

5

Passages

c-Jun

-/-

+/-

+/-

-/-

AA

-/-

AA

-/-

+/+

+/+

49

184

Actin

121

121

86

86

36

61

61

49

JNK

GST-

c-Jun

49

26

P-c-Jun

36

32

mkk7+/+

mkk7-/-

control

c-Jun

pBabe

control

121

86

61

c-Jun

49

36

32

121

86

61

49

Actin

36

32

a

b

c

The full scan for Fig. 4g

The full scan for Fig. 4f

The full scan for Fig. 6a

d

f

e

wt

JunAA

4

6

8

4

6

8 Passages

cdc2

28S

18S

The full scan for Fig. 6c

The full scan for Fig. 6b

The full scan for Fig. 7d

Penninger


  • Login