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Cutting the pGlo Plasmid with Restriction Enzymes to confirm Plasmid.

Cutting the pGlo Plasmid with Restriction Enzymes to confirm Plasmid. Kyle Schaf. Research Questions. Does the predicted enzyme cuts actually match the actual gel image? Do the predicted fragments match the actual fragments generated?. Hypotheses Tested.

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Cutting the pGlo Plasmid with Restriction Enzymes to confirm Plasmid.

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  1. Cutting the pGlo Plasmid with Restriction Enzymes to confirm Plasmid. Kyle Schaf

  2. Research Questions • Does the predicted enzyme cuts actually match the actual gel image? • Do the predicted fragments match the actual fragments generated?

  3. Hypotheses Tested • If my enzyme recognizes the predicted locations then………….. • If………….. Then……….. • Etc….

  4. Procedures • Do not print it out word for word. • Summarize. • Make sure to give the entire setup for the digestion.

  5. Results • Show the predicted gel image from NEB cutter • Show the dried gel image or image you took with the digital camera • Make sure to annotate the gel image showing the control lanes and sizes of fragments.

  6. Conclusion • What are your findings • Answer the questions • Areas of Weakness • New questions to pursue based on your findings.

  7. References • Websites used • People consulted • Partners • Books or journals used • Follow proper format

  8. Pointers for the format • White background with black text is easiest to see • 30 pt font is best or larger • Keep text to a minimum on each page • Pictures really help • Graphs and charts really help • Talk to the people not to the ppt slide. Don’t read to us……… we can read.

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