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Development of Antiserum Against Tomato Chlorosis Virus Coat Protein Expressed in Escherichia coli

This study focuses on the preparation of polyclonal antiserum against the coat protein (CP) of Tomato Chlorosis Virus (ToCV) expressed in Escherichia coli. Outbreaks of yellowing disease on tomato crops in Indonesia since 2006 have been observed, causing significant yield reduction. Detection of the viruses using RT-PCR confirmed the presence of ToCV and Tomato Infectious Chlorosis Virus (TICV) in affected plants. The antiserum developed in this study will aid in further research and development of management strategies for this devastating disease.

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Development of Antiserum Against Tomato Chlorosis Virus Coat Protein Expressed in Escherichia coli

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  1. PENYEDIAAN ANTISERUM POLIKLONAL TERHADAP COAT PROTEIN (CP) DARI TOMATO CHLOROSIS VIRUS (ToCV) YANG DIEKSPRESIKAN PADA Escherichia coli GedeSuastika & Giyanto DepartemenProteksiTanaman, FakultasPertanian, InstitutPertanian Bogor

  2. OUTBREAKS OF YELLOWING DISEASE ON TOMATO IN INDONESIA Since 2006, outbreaks of yellowing disease on tomato (Lycopersiconesculentum) crops have occurred in several regions of Indonesia. Especially in upland of Garut and Cipanas [West Java], Magelang [Central Java], Bedugul [Bali] and Sembalun [Lombok].

  3. OUTBREAKS OF YELLOWING DISEASE ON TOMATO IN INDONESIA Appearance of yellowing diseases affected tomato plants found in Garut [West Java], Magelang [Central Java], Bedugul [Bali] and Sembalun [Lombok].

  4. OUTBREAKS OF YELLOWING DISEASE ON TOMATO IN INDONESIA The disease was characterized by interveinal yellowing that developed initially on lower leaves and then progressed to the upper part of the plant. No obvious symptoms are produced on fruit, but significant yield reduction occurs.

  5. DETECTION OF THE VIRUSES BY USING RT-PCR The yellowing disease symptoms resembled to those described in USA, Spain and others counties for the infections caused by Tomato chlorosis virus (ToCV) and Tomato infectious chlorosis virus (TICV) Both viruses are belong to Family Closteroviridae; Genus Crinivirus Crinivirus has flexuous, filamentous particles [12 x 850-900 nm]; a bipartite, single-stranded RNA genome [RNA 1 and RNA 2 are 7.8 and 7.4 kb, respectively] (Duffuset al. 1996)

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