1 / 12

C o l o r i m e t r i c o f a s p i r i n determination

C o l o r i m e t r i c o f a s p i r i n determination. Acetyl salicylic acid. antiplatelet effect by inhibiting the production of thromboxane. purposes. d etermine the amount of aspirin in a commercial aspirin product.

pier
Download Presentation

C o l o r i m e t r i c o f a s p i r i n determination

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Colorimetric of aspirin determination

  2. Acetyl salicylic acid antiplatelet effect by inhibiting the production of thromboxane

  3. purposes • determine the amount of aspirin in a commercial aspirin product. • used to determine the purity of the aspirin produced in the Microscale Synthesis of Acetylsalicylic Acid lab.

  4. principle ExxNaOH ∆ + Na acetate Na salicylate Neutralize Why? By what ? Conc.HCL FeCL3 To prevent form Fe(OH)3 Colored complex (violet) Intensity of color direct relationship with substance concentration

  5. procedure Part I: making standard • Weight 0.4 g of acetyl salicylic acid (authentic powder) • and put in 100 ml beaker then Add 20 ml distilled water • then add 10 mL of a 0.5N NaOH then heat it on hot plate until • boiling then add 2 drops Ph.ph togive pink • color then add Conc. HCL to neutrlize

  6. Quantitatively transfer the solution to a 250 mL volumetric • flask, and dilute with distilled water to the mark.. • Pipet a 5mL of standard solution to a 100 mL volumetric flask. • then add 5 ml of FeCL3 solution then complete to market with • distilled water. • Label the flask .

  7. Part II: making sample • put one tablet in 100 ml beaker then add 20 ml distilled water by measure then add 5 ml of 0.5 N NaOH . • Heat it on hot plate until boiling the add 2 drops Ph.Ph. To give pink Color then add Conc. HCL to neutralize it . • Filtrate it onto volumetric flask 250 ml by using fitter paper and • Funnel then complete with • distilled water (mix well)

  8. Pipette 5ml of sample solution to 100 ml volumetric flask . • Add 5 ml of 5% FeCL3 solution then complete with distilled water To the market. ( mix well). • Label the flask. • Part III: making blank • Put 5 ml of FeCL3 to 100ml volumetric flask then complete • the volume by distilled water

  9. Measure the absorbance at λ max 510 nm Standard sample blank

  10. Calculation • for standard • concentration of standard is 8 mg/100 ml (mg%) • ᶜᶬ A 1%

  11. For sample Calculated Conc. Practical Conc. = 2 mg /100ml (mg%) 1-beers law. 2-matching point.

  12. % recovery (95-105) Practicale calculated According to the result we can say the tablet accepted or rejected • X100

More Related