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Bioproduct Refining

Bioproduct Refining. Biocatalytic Nanostructure. Biocatalytic Microstructure. Enabling Toolbox. Bioproduct Refinery. The ability of a biorefinery to achieve market economics will depend significantly on improvements in the accessibility of

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Bioproduct Refining

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  1. Bioproduct Refining Biocatalytic Nanostructure Biocatalytic Microstructure Enabling Toolbox

  2. Bioproduct Refinery The ability of a biorefinery to achieve market economics will depend significantly on improvements in the accessibility of the C-6, C-5 , and C-3 content of lignocellulose and hemicellulose and the ability to produce value-added products concurrently with ethanol, hydrogen, and biodiesel.

  3. Bioproduct Modification Pre-Harvest 1. Alteration of cellulose synthase 2. Replace cellulose content with starch content 3. Reduce lignin synthesis 4. Eliminate/reduce xylan side chain biosynthesis 5. Reduce phenylpropanoid biosynthesis 6. Eliminate reduce phenylpropanoid crosslinking

  4. Bioproduct Modification-Post Harvest 1. Improved cellulases xylanases 2. Seed-specific expression of heat tolerant and acid tolerant depolymerizing enzymes.

  5. Bioproduct Modification Post-Processing 1. Catalytic alkylation 2. Selective denitrification 3. Selective deoxygenation 4. Selective dehydration 5. Carboxylate reduction 6. Olefin oxidation 7. Olefin metathesis 8. Controlled cracking 9. Controlled reforming

  6. Biocatalytic Nanostructure 1. Stereoselectivity and regioselectivity vs basic catalytic activity 2. Dynamic vs. static structure features single molecule spectroscopy 3. Enzymology at interfaces 4. Metalloenzyme assembly 5. Prediction of protein and enzyme structure 6. Discovery of new enzymes 7. Improved enyzmes via rational design and directed evolution

  7. Biocatalytic Microstructure 1. Transcriptome/proteome analysis 2. Enzyme-enzyme, enzyme-membrane, enzyme-cell wall, enzyme-solute matrix 3. Small molecule import 4. Product export 5. Biocatalytic platforms 6. Metabolomics

  8. Enabling Toolbox 1. Promoters inducers host range mRNA Stability “all or none” 2. Transcription/Translation codon usage folding proteolysis 3. Gene shuffling genomic model assisted high throughput analysis

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