1 / 3

AGAUUUUAUUGUUAAUUUAGCUGCU UAUUUUUAAAGAUGUUUUUAUUUGU

A. -125. -100. -76. AGAUUUUAUUGUUAAUUUAGCUGCU UAUUUUUAAAGAUGUUUUUAUUUGU AAGAUCACGUGUUCGGGGUCAUGGU GUUUGUUACUUGUAUAAGUAUAAAU ACACCAAUAAAUCCUUCACAUUUCC. cdk9 MO-4. -26. -75. -51. -50. cdk9 MO-3. cdk9 MO-2. -25. -1. AAAAAAAAAAAAAAAAAA. cdk9 MO. B. cdk9 MO-2. cdk9 MO-3. cdk9 MO-4.

pennie
Download Presentation

AGAUUUUAUUGUUAAUUUAGCUGCU UAUUUUUAAAGAUGUUUUUAUUUGU

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. A -125 -100 -76 AGAUUUUAUUGUUAAUUUAGCUGCU UAUUUUUAAAGAUGUUUUUAUUUGU AAGAUCACGUGUUCGGGGUCAUGGU GUUUGUUACUUGUAUAAGUAUAAAU ACACCAAUAAAUCCUUCACAUUUCC cdk9MO-4 -26 -75 -51 -50 cdk9MO-3 cdk9MO-2 -25 -1 AAAAAAAAAAAAAAAAAA cdk9MO B cdk9MO-2 cdk9MO-3 cdk9MO-4 cdk9mMO (-) cdk9MO 3 hpf 500 cdk9 400 300 PAT1 500 tbp 400 300 1 2 3 4 5 6 C cdk9MO-2 cdk9MO-3 cdk9MO-4 cdk9mMO (-) cdk9MO HeLa NE 5 hpf anti-zCdk9 actin 1 2 3 4 5 6 7 Supplementary Figure 1

  2. A zcdk9 3’UTR GUUACUUGUAUAAGUAUAAAUACACCAAUAAAUCCUUCACAUUUCC poly(A) cdk9 MO TGTGGTTATTTAGGAAGTGTAAAGG ztbp 3’UTR poly(A) CUUUGCUACGUUGAACGCAAAUAAAUGUGUUUGCCAAGUUCUGCCUAU TTACACAAACGGTTCAAGACGGATA tbp MO zcyclin B1 3’UTR poly(A) GUUUAUGCAAGUUUGUACAUUUGAAUUCAAAUAAACUUUUUAAAGUUUU cyclin B1 MO TAAGTTTATTTGAAAAATTTCAAAA zcyclin B2 3’UTR poly(A) UUUUGACCUGUUAAAGGAACUUGAAUUCAUUAAAAUCCAAAGAUUCAGU cyclin B2 MO TAAGTAATTTTAGGTTTCTAAGTCA B cdk9MO cdk9mMO tbpMO cycB1MO cycB2MO WT hpf 2 3 4 5 2 3 4 5 2 3 4 5 2 3 4 5 2 3 4 5 2 3 4 5 PAT cdk9 oligo dT PAT tbp oligo dT PAT cyclin B1 oligo dT PAT cyclin B2 oligo dT tbp random actin random 17 18 19 20 21 22 23 24 12 13 14 15 16 1 2 3 4 5 6 7 8 9 10 11 Supplementary Figure 2

  3. A Template DNA in vitro transcription(T7 RNA Polymerase) C Elution ztbp 3’UTR zcdk9 3’UTR - - - - + + cdk9 MO - - - - + + tbp MO probe B zcdk9 3’UTR - - + cdk9 MO - - + cdk9m MO Supplementary Figure 3

More Related