Università degli Studi di Milano.
This presentation is the property of its rightful owner.
Sponsored Links
1 / 22

Università degli Studi di Milano. Member of LERU (League of European Research Universities). PowerPoint PPT Presentation


  • 54 Views
  • Uploaded on
  • Presentation posted in: General

Università degli Studi di Milano. Member of LERU (League of European Research Universities). 1° University in Italy for scientific productivity (Leiden ranking 2012). 1° University in Italy in Biomedical Sciences. Dipartimento di Bioscienze 90 Faculty members, 6 EMBO Members.

Download Presentation

Università degli Studi di Milano. Member of LERU (League of European Research Universities).

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript


Universit degli studi di milano member of leru league of european research universities

Università degli Studi di Milano.

Member of LERU (League of European Research Universities).

1° University in Italy for scientific productivity (Leiden ranking 2012).

1° University in Italy in Biomedical Sciences.

Dipartimento di Bioscienze

90 Faculty members, 6 EMBO Members.

Genetics, Molecular Biology, Structural Biology, Biochemistry, Bioinformatics, Developmental Biology, Physiology, Plant Biology, Ecology.

PhD program in Biomolecular Sciences.

50 students.

Joint PhD program with EMBL, Heidelberg.


Universit degli studi di milano member of leru league of european research universities

Progenitor

cells

Pancreatic

cell

Endoderm

Hepatocyte

Myoblast

Mesoderm

B cell

Totipotent cell

Neuron

Ectoderm

Epithelial cell

Pluristratified

ES cell


Universit degli studi di milano member of leru league of european research universities

A1 helix

K261

P263

F50

E52

Y56

R266

E82

Q85

E86

R249

R253

I117

E90

S93

S97

D116

F113

R250

R245

F94

Q110

I246

E98

F125

V238

D112

E101

L123

T122

Q242

D109

S121

K241

N239

T

A

A

C

A2

C

a2(YB)

a1(YC)

aC(YC)

A1

NF-Y-DNA structure

  • NF-YA/NF-YB/NF-YC interface

  • A1 helix contacts extensively NF-YB/NF-YC (1760 Å2)

  • heterotrimerization through side-chain and backbone polar interactions

  • A1A2-linker: extended conformation that directs A2 helix towards the DNA


Universit degli studi di milano member of leru league of european research universities

The genomic strategy of NF-Y:

The ENCODE

Project.

ChIp-Seq/Mass-Spec in 9 cell types of

10 histone PTMs and 72 -and counting- DNA-binding Proteins (Mostly TFs).


Universit degli studi di milano member of leru league of european research universities

Clustering NF-Y with 80 nuclear proteins.


Universit degli studi di milano member of leru league of european research universities

40/50 TFs show correlations,

P value E-40


Universit degli studi di milano member of leru league of european research universities

NF-Y friends

GTFs

TFs

Cofactors


Universit degli studi di milano member of leru league of european research universities

NF-Y and friends

stay together...


Universit degli studi di milano member of leru league of european research universities

...to make life and death decisions

cFos

cJun

NFYB

RefSeq


Universit degli studi di milano member of leru league of european research universities

5’

T

A

-1

1

T

C

G

A

Gln 97

Arg 93

L2 (C)

-2

2

C

G

G

G

G

G

C

C

C

C

Arg 94

Thr 95

C

G

-3

3

L1 (B)

Lys 75

T

A

-4

T

T

A

A

4

a2 (B)

Lys 78

-5

5

Ala 77

A

T

L1 (B)

Ile 76

-6

6

A

A

T

T

Lys 75

a2 (B)

G

C

Lys 81

-7

7

Gly 287

-8

Phe 289

Met 279

8

Gly 286

Arg 288

A2

-9

9

His 275

Arg 283

Ser 291

His 271

-10

10

A1A2-linker

Leu 270

Arg 281

Pro 58

-11

11

Ile 59

His 277

Ala 60

a1 (B)

-12

12

Asn 61

A2

Arg 64

C

G

-13

13

Arg 274

Ser 273

A

T

A1A2-linker

-14

14

His 271

C

G

Gln 53

-15

15

Lys 50

Arg 47

a1 (C)

C

G

-16

16

Ala 46

Leu 45

Pro 44

-17

17

A

T

-18

18

-19

19

C

G

-20

20

Met 60

Lys 53

-21

21

a1 (C)

Lys 49

L1 (C)

Ile 61

C

G

Ser 62

-22

22

Ala 63

-23

23

A

T

-24

24

L2 (B)

Lys 109

T

A

-25

25

5’

The amazing precision of ChIP-Seq


Universit degli studi di milano member of leru league of european research universities

Where do the friends sit at the table?

?

CCAAT

?

E2F

E box

TBP

AP1


Universit degli studi di milano member of leru league of european research universities

We can tell


Universit degli studi di milano member of leru league of european research universities

Reconstructing promoters architecures

10

50

CCAAT

E2F

E box

TBP

AP1


Universit degli studi di milano member of leru league of european research universities

Protein transfections

of TAT-NF-YA in vivo.

Domashenko, Blood, 2010.

Progenitor

cells

Pancreatic

cell

Endoderm

Hepatocyte

ES cell

Myoblast

Mesoderm

Hematopoietic

cells

HSCs

Totipotent cell

Neuron

Ectoderm

Epithelial cell

Pluristratified


Universit degli studi di milano member of leru league of european research universities

-LIF +TAT-NF-YAs

UNTREATED

-LIF +TAT

-LIF

VINCULIN

TAT-NF-YAs

NF-YAl

NF-YAs

SOX2

NANOG

KLF5

Fold enrichment


Universit degli studi di milano member of leru league of european research universities

TFBs analysis on Nanog core promoters

Nanog peaks de novo discovery analysis

Mouse promoters Nanog peaks

Ranking positions third

Human promoters Nanog peaks

Ranking positions first

Mouse

Human

82.2

ChIP:NF-YB

89.3

Fold enrichment

NF-YAs

NF-YC

ChIP:NANOG

NF-YAsm29

Fold enrichment

NF-YC


Universit degli studi di milano member of leru league of european research universities

NF-YC

NF-YB

NF-YA

A growing network of TFs in ESC.

37

Short

KLF4

SALL4

MYC

STAT3

OCT4

SOX2

NANOG


Universit degli studi di milano member of leru league of european research universities

Past

DSBB U. Milano

Post-Docs

Khalid Zemzoumi

Alberto Di Silvio (Axxam I)

Fabienne Cocchiarella (UniMo)

Marià Pitarque Martì

Anna Testa

Nicoletta Cordani (HSR, I)

Silvia Pozzi (Reg Emilia)

Alessandra Viganò (U. Basel, CH)

PhDs

Maria Carla Motta (U. Manchester UK)

Patricia Lievens

Chiara Liberati (Axxam, I)

Giuseppina Caretti (UniMi I)

Mattia Frontini (MRC UK)

Carol Imbriano (UniMo I)

Barbara Testoni (CNRS F)

Claudia Forni (Farmigea I)

Giacomo Donati (MRC UK)

Michele Ceribelli (NIH USA)

Daniele Merico (U. Toronto CA)

Andrea Fossati (UniMi I)

Marianna Bellorini

Chiara Vecchi (UniMo I)

Valentina Salsi (UniMo I)

Giulia Petrovich (U. Edimburgh UK)

Present

Collaborators

Serena Borrelli

Raffaella Gatta

Diletta Dolfini

Elena Martynova

Mario Minuzzo

Nerina Gnesutta

Mariangela Lorenzo

Federico Zambelli

Giulio Pavesi

Marco Nardini

Martino Bolognesi

C ImbrianoU Modena (I)

L ToraIGBMC (F)

C RomierIGBMC (F)

C CastagnoliB.Cute Torino (I)

G MelinoMRC Leicester (I)

A Terrinoni IDI (I)

E CandiU. Roma (I)

D AberdamINSERM-Nice (F)

GP DottoU. Lausanne (CH)

E BertiU. Mi-Bicocca (I)

M d’IncalciIst Mario Negri (I)

D. HochhauserUCL (UK)

J. Fleming/K. StruhlHarvard (USA)

Active Motif (USA)


  • Login