Evaluation of candidate standard xx 97 650
This presentation is the property of its rightful owner.
Sponsored Links
1 / 8

Evaluation of Candidate Standard XX (97/650) PowerPoint PPT Presentation


  • 75 Views
  • Uploaded on
  • Presentation posted in: General

Evaluation of Candidate Standard XX (97/650). Clare Davis Division of Retrovirology National Institute for Biological Standards and Control (NIBSC) UK. 1 st International Standard for HIV-1 RNA (NIBSC code 97/656). Established as International Standard in 1999 ( Holmes et al, 2001).

Download Presentation

Evaluation of Candidate Standard XX (97/650)

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript


Evaluation of candidate standard xx 97 650

Evaluation of Candidate Standard XX (97/650)

Clare Davis

Division of Retrovirology

National Institute for Biological Standards and Control

(NIBSC)

UK

SoGAT Paris 2004


1 st international standard for hiv 1 rna nibsc code 97 656

1st International Standard for HIV-1 RNA (NIBSC code 97/656)

  • Established as International Standard in 1999 (Holmes et al, 2001).

  • Assigned a concentration of 100,000 IU per vial/ml.

  • Based on HIV-1 PCR positive, antibody negative plasmapheresis donation.

  • Virus diluted 1:312.5 in base matrix (defibrinated plasma).

  • 2400 vials freeze-dried. Held at -200C.

  • Preparation showed good stability and correlation amongst assays.

  • Discussions at SoGAT – Jul 2003 (PEI) raised:

    • Low Stocks (~ 650).

    • Presence of HBV DNA – problematical for multiplex assays.

SoGAT Paris 2004


Evaluation of candidate standard xx 97 650

--- bDNA

--- Monitor

--Nuclisens

--- Abbott

--- in-house

SoGAT Paris 2004


Re evaluation of candidate xx

Re-evaluation of Candidate XX

  • Non-syncitium inducing HIV-1 genotype B field isolate field isolate (donated by Dr. P. Simmonds).

  • Supplied as a low passage virus.

  • Further propagated on human PBMC’s, supernatant stored in LN2.

  • Virus diluted 1:250 in pooled human plasma.

  • 2200 vials of material freeze-dried.

SoGAT Paris 2004


Study details

Study Details

  • Study purpose:-

    • Calibrate XX against HIV-1 RNA International Standard.

    • Confirm stability of XX

    • Determine values in current assays

  • 9 Laboratories participating.

  • International Standard and Candidate XX randomly coded.

  • Participants asked to test samples in 3 independent assays.

    • Initially 10 fold dilution

    • Then 2 subsequent assays half log dilutions around the end point.

  • NIBSC to analyse data.

  • SoGAT Paris 2004


    Preliminary analysis of data so far quantitative

    Preliminary analysis of data so far…..Quantitative

    Values obtained in previous study 5.31 4.73

    SoGAT Paris 2004


    Qualitative

    Qualitative

    Values obtained in previous study 4.70 4.22

    SoGAT Paris 2004


    Conclusion

    Conclusion

    • Preliminary analysis of data shows good correlation with previous study.

    • Hope to submit report to ECBS Aug 04.

    • Please return any outstanding data to us by end Jun 04.

    SoGAT Paris 2004


  • Login