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PI-PLC1-CYFP+PI-PLC1-NYFP

A1. A2. PI-PLC1-CYFP+PI-PLC1-NYFP. PI-PLC1-CYFP +PI-PLC1-NYFP. B2. B1. B3. PI-PLC1+Gα-Q223L. ER-mCherry. Merge. C1. C2. C3. PI-PLC1+Gα-Q223L. PM-mCherry. Merge. D1. C2. C1. PI-PLC1-CYFP +Clo3-NYFP. Figure S8. BiFC interactions in tobacco leaf epidermal cells.

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PI-PLC1-CYFP+PI-PLC1-NYFP

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  1. A1 A2 PI-PLC1-CYFP+PI-PLC1-NYFP PI-PLC1-CYFP +PI-PLC1-NYFP B2 B1 B3 PI-PLC1+Gα-Q223L ER-mCherry Merge C1 C2 C3 PI-PLC1+Gα-Q223L PM-mCherry Merge D1 C2 C1 PI-PLC1-CYFP +Clo3-NYFP Figure S8. BiFC interactions in tobacco leaf epidermal cells. A: PI-PLC1-CYFP and PI-PLC1 -NYFP dimerization on ER and PM; B:co-localization of GA3-Q223L-CYFP and PI-PLC1-NYFP interaction to ER; C:co-localization of GA3-Q223L-CYFP and PI-PLC1-NYFP to PM; Twenty cells of GA3-Q223L-CYFP and PI-PLC1-NYFP BiFC interaction have higher YFP flourecent compered to GA3-CYFP and PI-PLC1-NYFP BiFC interaction; D:the localization of the interaction between the two PI-PLC1-CYFP and Clo3-NYFP apparent by ER network structure seen in the upper focal planes of the cell. Scale bar = 24 µM

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