Metacaspase. Single or Multicopy gene in Emiliania huxleyi ?. Isolation of Genomic DNA from Emiliania huxleyi. CTAB Isolation and CsCl Gradient. Research Plan. Isolate Genomic DNA. Southern Blot Analysis. Digest Genomic DNA w/ Various Restriction Enzymes.
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Single or Multicopy gene in Emiliania huxleyi?
CTAB Isolation and CsCl Gradient
Isolate Genomic DNA
Southern Blot Analysis
Digest Genomic DNA w/ Various Restriction Enzymes
Agarose Gel Electrophoresis and Southern Transfer
Make Non-Radioactive Metacaspase Probe
Hyribidize Probe to Southern Blot
Washes and Chemiluminescent Detection
To Isolate High Quality Genomic DNA from Emiliania huxleyi
Breaking open E. huxleyi cells
Removal of other Cellular Macromolecules
Cesium Chloride Gradient
Ultraviolet Spectroscopic Analysis of Nucleic Acids