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Supplementary Figure 7 Hayashi et al PowerPoint PPT Presentation


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Control. Treatment. 4 x 44K custom oligo -DNA microarray chip ( Catalog number G2514F: Agilent Technologies, Palo Alto, CA; GEO ID: GPL 7252 ) [ G3 Scanner, AGILENT]. Healthy at NIES and ITF (small exposure on the way to ITF). Exposure to Low-level Gamma Radiation at ITF. C-0h. T-6h.

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Supplementary Figure 7 Hayashi et al

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Supplementary figure 7 hayashi et al

Control

Treatment

4 x 44K custom oligo-DNA microarray chip

(Catalog number G2514F: Agilent Technologies, Palo Alto, CA; GEO ID: GPL 7252)

[G3 Scanner, AGILENT]

Healthy at NIES and ITF (small exposure on the way to ITF)

Exposure to Low-level Gamma Radiation at ITF

C-0h

T-6h

T-72h

C-72h

6-10 seedlings each = Pooled 3rd Leaves

C-0h

T-72h

C-0h

T-6h

Leaf

NIES

NIES

ITF – Iitate Farm, Iitate Village, Fukushima

NIES – National Institute for Environmental Studies

C-0h

T-72h

C-0h

T-6h

Differentially

Expressed

Genes - ITF

Cy3

×

×

Chip 1

Dye-Swap

Cy5

Differentially

Expressed

Genes – Control

(0 h – early morning changes)

(72 h – growth effects at NIES)

C-0h

NIES

C-0h

C-0h

NIES

C-72h

NIES

Cy3

×

×

Chip 2

Dye-Swap

C-0h

C-0h

NIES

C-72h

NIES

C-0h

NIES

Cy5

Supplementary Figure 7. DNA microarray chip/slide design and placement of control and treatment sample sets using the dye-swap approach. Two 4x44K chips were used in this study.

Supplementary Figure 7

Hayashi et al


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