Isolation of Caffeine from Tea Leaves. Caffeine. Alkaloid Purine Ring System Stimulant (50 – 200 mg) Diuretic. Caffeine. Found in natural sources Coffee Tea Kola nuts Cocoa beans. Tea. Caffeine 5% by weight Soluble in hot water Tannins High MW phenolic compounds Soluble in water
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Isolation of Caffeine from Tea Leaves
Open two tea bags and weigh contents to the nearest 0.0001g.
Put tea into one bag, close, and staple.
Carefully add 5.0 mL of dichloromethane (DCM) to the separatory funnel.
Follow the procedure in the lab manual CAREFULLY (pg. 89 step 5).
Your instructor will now demonstrate the proper extraction procedure using the separatory funnel!
What is in the top layer?
The bottom layer??