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Apc Min /APRIL-Tg

Supplementary Figure 1. Ectopic expression of APRIL promotes adenoma formation in Apc Min mice. Analysis per section of the intestinal tract. *. *. 80. 60. Number of adenomas. 40. 20. 0. Apc Min. Apc Min. Apc Min. Apc Min. Apc Min /APRIL-Tg. Apc Min /APRIL-Tg.

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Apc Min /APRIL-Tg

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  1. Supplementary Figure 1.Ectopic expression of APRIL promotes adenoma formation in ApcMin mice. Analysis per section of the intestinal tract * * 80 60 Number of adenomas 40 20 0 ApcMin ApcMin ApcMin ApcMin ApcMin /APRIL-Tg ApcMin /APRIL-Tg ApcMin/APRIL-Tg ApcMin /APRIL-Tg Duodenum Jejunum Ileum Colon

  2. ns ns ns of tumor tissue of tumor tissue of tumor tissue Number of positive cells Number of positive cells Number of positive cells 2 2 2 per mm per mm per mm 200 150 100 50 0 apcMIn apcMIn/APRIL-Tg Supplementary Figure 2. ApcMIn and apcMin/APRIL-Tg mice present similar levels of tumor infiltration levels ApcMin/APRIL-Tg ApcMin 150 100 CD3 50 0 apcMIn apcMIn/APRIL-Tg 50 40 B220 30 20 10 0 apcMIn apcMIn/APRIL-Tg Ly6 F480

  3. Supplementary Figure 3. APRIL inducible knock-down. Correlation between RFP expression and APRIL Knock-down. + dox 100 - dox 75 50 % RFP positive cells 25 0 days 0 1 3 6 9 14 APRIL Erk

  4. Supplementary Figure 4. APRIL knockdown affects clonogenicity. Expansion with a second shRNA targeting APRIL (shRNA17) A A 60 60 40 40 Clonogenicity Clonogenicity 20 20 0 0 APRIL KD (17) I APRIL KD (17) I APRIL KD (17) I APRIL KD (17) I APRIL KD(17) NI APRIL KD(17) NI APRIL KD(17) NI APRIL KD(17) NI APRIL KD(18) I + mock APRIL KD(18) NI + Mock APRIL KD(18) NI + APRIL APRIL KD(18) I + HSPG-T APRIL KD(18) I + APRIL wt Ls180 HT-29 HCT-81 DLD-1 Supplementary Figure 5. APRIL expression levels on mice colorectal cell lines and APRIL KD 0.015 0.010 0.005 (relative to 18S) mRNA expression 2e-006 2e-006 2e-006 1e-006 5e-007 0 CT26 Splenocytes CMT93-Control CMT93-APRIL KD

  5. -04 8. 10 -04 6. 10 -04 4. 10 -04 -06 -04 -04 -04 -07 3. 2. 2. 4. 1. 1. 10 10 10 10 10 10 -04 2. 10 -05 -05 -05 -04 -05 -05 -06 1. 1. 6. 5. 3. 4. 2. 10 10 10 10 10 10 10 0 NIH A20 Testis untreated Splenocytes DSS treated Supplementary Figure 6. APRIL receptor expression in mice models and human colorectal cell lines. TACI expression in C57BL/6 mice BCMA expression in C57BL/6 mice 0.0008 0.0003 0.0006 0.0002 0.0004 mRNA expression (relative to 18S) mRNA expression (relative to 18S) 0.0001 0.0002 -07 4. 10 0 0 NIH NIH A20 A20 Testis Testis untreated untreated Adenoma1 Adenoma1 Splenocytes DSS treated Splenocytes DSS treated TACI expression in BALB/c mice BCMA expression in BALB/c mice mRNA expression (relative to 18S) mRNA expression (relative to 18S) 0 NIH A20 Testis untreated Splenocytes DSS treated TACI expression in human BCMA expression in human CRC cell lines CRC cell lines 0.050 0.0020 0.040 0.0016 0.030 0.0012 mRNA expression relative to GAPDH relative to GAPDH mRNA expression 0 0 RKO L363 RKO L363 HT-29 LS180 DLD-1 HT-29 LS180 DLD-1 SW837 SW480 SW620 HCT-81 HCT-15 SW837 SW480 SW620 HCT-81 HCT-15 HCT-116 HCT-116

  6. Supplementary Figure 7. HSPG expression on human and mouse CRC cell lines. Ls180 HT-29 HCT-81 Sw480 DLD-1 PE 10E4 Isotype control Co1 Co2 PE CMT93 C26 PE

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