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Filippo Venezia Diplom biologist (t.o.) ZBSA Superviser: Dr. Fleck Prof. Schaefer

Modeling of phyB-dimers ( indicates that PfrPfr homodimers are the dominant signalling components ). Filippo Venezia Diplom biologist (t.o.) ZBSA Superviser: Dr. Fleck Prof. Schaefer. Dimer model. P fr -P fr - D 2. P fr -P r - D 1. P r -P r - D 0. k 1 =.

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Filippo Venezia Diplom biologist (t.o.) ZBSA Superviser: Dr. Fleck Prof. Schaefer

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  1. Modeling of phyB-dimers(indicatesthatPfrPfr homodimers arethe dominant signallingcomponents) Filippo Venezia Diplom biologist (t.o.) ZBSA Superviser: Dr. Fleck Prof. Schaefer

  2. Dimer model Pfr-Pfr - D2 Pfr-Pr - D1 Pr-Pr - D0 k1 = N Photo-conversion rate k2 = N ks = synthesis kdr,fdr= degradation kr = dark reversion

  3. Simplified phyB pooldimermodel Whicharethephysiologicalfunctionalpool? Whicheffectshavethenewunknownparameters on thesystem? New unknownparameters: k3*, k4*, kr*

  4. Model selection Pfr-Pfr Pfr total Red = 699nm Indigo = 692nm Black = 687nm Blue = 681nm Green = 672nm Poolkombination: RSS X2 AIC BIC F-test D2ns + D2n0.06130.09-57.26-59.67 1.083 D1ns + D1n 0.972 3.563 -27.25 -29.66 1.81 D1+ D2 0.0895 0.128 -53.15 -55.56 1.118 (Pfr-Pool) f0.9=1.69 n=20

  5. Approximation to darkreversion experimental data n=10 Identificable Parameter kr = 1.5333 krr = 0.0989 X2 = 0.0158 kr/krr = 15.5 Indigo = OhrGFP/A-B- Blue = ABO/A- on ice Red = calc. Withoutdarkreversion Black = fittedwithdarkreversion Green = ABO/A-

  6. Approximation withoutspeckles Red = 699nm Indigo = 692nm Black = 687nm Blue = 681nm Green = 672nm Model withoutspecklescannotfit thedata

  7. Summary • Dimermodelcandescribephoton-fluxresponsecurve • sufficiently • The optimal Model explainingPfr-Pfrhomodimer dataisobtainedthroughtheuse of selectionmethods. • Specklesare essential forstabilizingdarkreversion • phyB dimers showsindividualdarkreversionkinetics.

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