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Medical Biochemistry Review #2 By Jason Elmer [email protected] Obi Ekwenna [email protected] YOUR EXAM Lectures 14-24 ~44 questions (4 questions per lecture) Take a calculator to the exam Exam on Monday October 4 th . DO THE STUDY QUESTIONS; if nothing else read the answers!!!!!!!!!!

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Medical biochemistry review 2 by l.jpg

Medical BiochemistryReview #2By

Jason Elmer

[email protected]

Obi Ekwenna

[email protected]


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YOUR EXAM

Lectures 14-24

~44 questions (4 questions per lecture)

Take a calculator to the exam

Exam on Monday October 4th.

DO THE STUDY QUESTIONS; if nothing else read the answers!!!!!!!!!!

Of course TLEs are highly recommended!


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It is impossible to memorize every possible bit of biochemistry trivia. ‘They’ simply know way too much about metabolism for a single person to be able to regurgitate it all.


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Do not biochemistry trivia. ‘They’ simply know way too much about metabolism for a single person to be able to regurgitate it all. rely on passive reading and highlighting/underlining of the textbook.

Do not sit and stare at the handouts

Do not try to read 50 review books. (Make your own review book instead!)

Do focus on identifying key concepts

Do actively draw and redraw pathways and connections

Do learn to identify relevant information


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Do prioritize: biochemistry trivia. ‘They’ simply know way too much about metabolism for a single person to be able to regurgitate it all.

What is the purpose of a pathway?

What are the starting and ending molecules?

Where is the pathway (in the cell, in a tissue, in an organ system)?

How does the pathway connect to other pathways?

What metabolic conditions turn the pathway on and off?

What are the control points for regulating the pathway?

reactants, products and enzyme name of each regulatory step

additional regulatory molecules involved (vitamins, cofactors)

make sure you know every step that makes or uses ATP

What structural features are important for the function and interaction of specific regulatory molecules in a pathway?

What biochemical techniques are used to study these pathways?

What specific drugs or diseases associated with the pathway?


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METABOLIC PATHWAYS biochemistry trivia. ‘They’ simply know way too much about metabolism for a single person to be able to regurgitate it all.

Glycolysis

Gluconeogenesis

Citric Acid Cycle (Krebs Cycle)

Glycogen Metabolism

Hexose Interconversions

Electron Transport Chain

Oxidative Phosphorylation

Pentose-Phosphate Shunt


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GLYCOLYSIS biochemistry trivia. ‘They’ simply know way too much about metabolism for a single person to be able to regurgitate it all.

Oxidation of glucose is known as Glycolysis.

Either AerobicPyruvate

Anaerobic Lactic Acid

Occurs in the Cytosol

Overall Rxn:

Glucose + 2 ADP + 2 NAD+ + 2 Pi  2 Pyruvate + 2 ATP + 2 NADH + 2 H+

NADH generated during glycolysis is used to fuel mitochondrial ATP synthesis via oxidative phosphorylation. Does not pass through mitochondrial membrane

2 ATP generatedglycerol phosphate shuttle

3 ATP generatedmalate-aspartate shuttle

If used to transport the electrons from cytoplasm NADH into the mitochondria.


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Key Reactions biochemistry trivia. ‘They’ simply know way too much about metabolism for a single person to be able to regurgitate it all.

Hexokinase

Found in the cytosol of most tissues

Low specificity: it’s a “hoe” for hexoses

Low Km: high affinity for glucose

Inhibited by Glucose-6-phosphate

Glucokinase: Found in the Liver and pancreatic b cells

Also a ‘hexokinase’

High specificity for glucose

High Km

inhibited by fructose-6-phosphate


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Regulation of Glycolysis biochemistry trivia. ‘They’ simply know way too much about metabolism for a single person to be able to regurgitate it all.

Hexokinase, PFK-1 and PK all proceed with a relatively large free energy decrease. These non-equilibrium reactions of glycolysis would be ideal candidates for regulation of the flux through glycolysis.

Hexokinase is not key because of G6P is generated by glycogenolysis

PK reaction is reversed in Gluconeogenesis

Therefore rate limiting step in glycolysis is the reaction catalyzed by PFK-1.

PFK-1 is a tetrameric enzyme that exist in two conformational states termed R and T that are in equilibrium.

ATP is both a substrate and an allosteric inhibitor of PFK-1. F6P is the other substrate for PFK-1 and it also binds preferentially to the R state enzyme. ATP binds the T state.


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The biochemistry trivia. ‘They’ simply know way too much about metabolism for a single person to be able to regurgitate it all.inhibition of PFK-1 by ATP is overcome by AMP which binds to the R state of the enzyme and, therefore, stabilizes the conformation of the enzyme capable of binding F6P.

The most important allosteric regulator of both glycolysis and gluconeogenesis is fructose 2,6-bisphosphate, F2,6BP, which is not an intermediate in glycolysis or in gluconeogenesis.

Also important to note that Insulin/Glucagon ratio i.e. fed/starve state, regulate Pyruvate Kinase activity. The last enzyme in the pathway.

Glucagon: high in starvation, b/cos blood glucose levels are low, therefore it favors gluconeogenesis in Liver.

Insulin: on the contrary favors glycolysis.


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Glycolysis biochemistry trivia. ‘They’ simply know way too much about metabolism for a single person to be able to regurgitate it all.


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Glycolysis biochemistry trivia. ‘They’ simply know way too much about metabolism for a single person to be able to regurgitate it all.

Key points about the Shuttle System:

Malate-Asparate shuttle is the primary system

By default Glycerol shuttle is secondary

Two enzymes are involved in this shuttle:

1.cytosolic version of the enzyme glycerol-3-phosphate dehydrogenase (glycerol-3-PDH) which has as one substrate, NADH.

2.mitochondrial form of the enzyme which has as one of its' substrates, FAD+. Since the electrons from mitochondrial FADH2 feed into the oxidative phosphorylation pathway at coenzyme Q (as opposed to NADH-ubiquinone oxidoreductase [complex I]) only 2 moles of ATP will be generated from glycolysis. G3PDH is glyceraldehyde-3-phoshate dehydrogenase.


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Glycolysis biochemistry trivia. ‘They’ simply know way too much about metabolism for a single person to be able to regurgitate it all.


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Malate -Asp Shuttle biochemistry trivia. ‘They’ simply know way too much about metabolism for a single person to be able to regurgitate it all.

The electrons are "carried" into the mitochondria in the form of malate. Cytoplasmic malate dehydrogenase (MDH) reduces oxaloacetate (OAA) to malate while oxidizing NADH to NAD+

Cytoplasmic malate dehydrogenase (MDH) reduces oxaloacetate (OAA) to malate while oxidizing NADH to NAD+.

Malate then enters the mitochondria where the reverse reaction is carried out by mitochondrial MDH

mitochondrial OAA goes to the cytoplasm to maintain this cycle ; must be transaminated to aspartate (Asp) with the amino group being donated by glutamate (Glu). The Asp then leaves the mitochodria and enters the cytoplasm. The deamination of glutamate generates a-ketoglutarate (a-KG) which leaves the mitochondria for the cytoplasm.

When the energy level of the cell rises, the rate of mitochondrial oxidation of NADH to NAD+ declines and therefore, the shuttle slows.


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The synthesis of biochemistry trivia. ‘They’ simply know way too much about metabolism for a single person to be able to regurgitate it all.F2,6BP is catalyzed by the bifunctional enzyme phosphofructokinase-2/fructose-2,6-bisphosphatase (PFK-2/F-2,6-BPase).

In the nonphosphorylated form the enzyme is known as PFK-2 and serves to catalyze the synthesis of F2,6BP by phosphorylating fructose 6-phosphate.

The result is that the activity of PFK-1 is greatly stimulated and the activity of F-1,6-BPase is greatly inhibited. More glycolysis!

When the bifunctional enzyme is phosphorylated it no longer exhibits kinase activity, but a new active site hydrolyzes F2,6BP to F6P and inorganic phosphate.

This enzyme is regulated by ProteinKinase A, which is a cyclic AMP dependent enzyme. cAMP is generated depending on the hormonal changes in the body. Eg. With Glucagon, high cAMP thus PKA is active thus less glycolysis.

In addition to these Pyruvate Kinase is activated by F1,6BP and inhibited by ATP.


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G biochemistry trivia. ‘They’ simply know way too much about metabolism for a single person to be able to regurgitate it all.luconeogenesis


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  • Substrates for Gluconeogenesis: biochemistry trivia. ‘They’ simply know way too much about metabolism for a single person to be able to regurgitate it all.Lactate, pyruvate, glycerol, propionny-CoA and certain Amino Acids but never FAT!!!

    • The Cori cycle involves the utilization of lactate, produced by glycolysis in non-hepatic tissues, (such as muscle and erythrocytes) as a carbon source for hepatic gluconeogenesis. In this way the liver can convert the anaerobic byproduct of glycolysis, lactate, back into more glucose for reuse by non-hepatic tissues. Note that the gluconeogenic leg of the cycle (on its own) is a net consumer of energy, costing the body 4 moles of ATP more than are produced during glycolysis. Therefore, the cycle cannot be sustained indefinitely.


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The glucose-alanine cycle is used primarily as a mechanism for skeletal muscle to eliminate nitrogen while replenishing its energy supply. Glucose oxidation produces pyruvate which can undergo transamination to alanine. This reaction is catalyzed by glutamate-pyruvate transaminase, GPT (also called alanine transaminase, ALT in Figure).


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Regulation of Gluconeogenesis for skeletal muscle to eliminate nitrogen while replenishing its energy supply. Glucose oxidation produces pyruvate which can undergo transamination to alanine. This reaction is catalyzed by

See regulation of Glycolysis via F2,6 P

Do not forget Hormonal regulations: Insulin and Glucagon

Other things to keep in mind

Pyruvate carboxylase is present in mitochondria, requires Biotin as a cofactor to convert Pyruvate OAA

MDH present in mitochondria, OAA to malate, then MDH present in cytosol converts malate back to OAA

OAA is then converted to PEP, as shown in the previous slide.

Pyruvate Carboxylase: inhibited by ADP and activated Acetyl CoA

PEP Carboxykinase in the cytosol is inhibited by ADP


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TCA /Citric Acid/KREBS Cycle for skeletal muscle to eliminate nitrogen while replenishing its energy supply. Glucose oxidation produces pyruvate which can undergo transamination to alanine. This reaction is catalyzed by

The cycle is located in the mitochondria

All cells have a mitochondria except RBCs

This is the Final common pathway of oxidative metabolism

Acetyl coenzyme A condenses with OAA to begin the cycle. Catabolism of CHO, Fats and Proteins provide the acetyl CoA

The bulk of ATP used by many cells to maintain homeostasis is produced by the oxidation of pyruvate in the TCA cycle

During this oxidation process, reduced NADH and reduced FADH2 are generated. The NADH and FADH2 are principally used to drive the processes of oxidative phosphorylation, which are responsible for converting the reducing potential of NADH and FADH2 to the high energy phosphate in ATP


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The PDH complex requires for skeletal muscle to eliminate nitrogen while replenishing its energy supply. Glucose oxidation produces pyruvate which can undergo transamination to alanine. This reaction is catalyzed by 5 different coenzymes: CoA, NAD+, FAD+, lipoic acid and thiamine pyrophosphate (TPP) . Three of the coenzymes of the complex are tightly bound to enzymes of the complex (TPP, lipoic acid and FAD+) and two are employed as carriers of the products of PDH complex activity (CoA and NAD+).pyruvate + CoA + NAD+  CO2 + acetyl-CoA + NADH + H+


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The TCA cycle showing enzymes, substrates and products. The abbreviated enzymes are: IDH = isocitrate dehydrogenase and a-KGDH = a-ketoglutarate dehydrogenase. The GTP generated during the succinate thiokinase (succinyl-CoA synthetase) reaction is equivalent to a mole of ATP by virtue of the presence of nucleoside diphosphokinase. The 3 moles of NADH and 1 mole of FADH2 generated during each round of the cycle feed into the oxidative phosphorylation pathway. Each mole of NADH leads to 3 moles of ATP and each mole of FADH2 leads to 2 moles of ATP.


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Overall Stoichiometry of TCA abbreviated enzymes are: IDH =

acetyl-CoA + 3NAD+ + FAD + GDP + Pi + 2H2O ----> 2CO2 + 3NADH + FADH2 + GTP + 2H+ + HSCoA

The GTP generated by Succinyl CoA SYNTHETASE IS VIA SUBSTRATE LEVEL PHOSPORYLATION.

Regulation of TCA:Regulation of the TCA cycle like that of glycolysis, occurs at both the level of entry of substrates into the cycle as well as at the key reactions of the cycle. Fuel enters the TCA cycle primarily as acetyl-CoA. The generation of acetyl-CoA from carbohydrates is a major control point of the cycle. This is the reaction catalyzed by the PDH complex

PDH complex is inhibited by acetyl-CoA, ATP, and NADH

PDH activated by non-acetylated CoA (CoASH) and NAD+.

The pyruvate dehydrogenase activities of the PDH complex are regulated by their state of phosphorylation. This modification is carried out by a specific kinase (PDH kinase) and the phosphates are removed by a specific phosphatase (PDH phosphatase).

The phosphorylation of PDH inhibits its activity which leads to decreased oxidation of pyruvate.

PDH kinase is activated by NADH and acetyl-CoA and inhibited by pyruvate, ADP, CoASH, Ca2+ and Mg2+. The PDH phosphatase, in contrast, is activated by Mg2+ and Ca2+

Citrate Synthase: inhibited by ATP and citrate

Isocitrate Dehydrogenase: Isocitrate, AMP, ADP activates, ATP and NADH inhibits

A-ketoglutarate dehydrogenase: succinoyl CoA and NADH inhibits

CindyIsKinkySoSheFornicatesMoreOften


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ELECTRON TRANSPORT AND OXIDATIVE PHOSPHORYLATION abbreviated enzymes are: IDH =

  • Each turn of TCA cycle generates 3NADH and 1 FADH2

  • Electron transport and oxophos occurs in the mitochondria

  • NADH and FADH2 ultimately pass electrons to O2 and produce H2O.

    • NADH  +  (1/2)O2  +  H+  -->NAD+  +  H2O ~ -52.6kcal/mol

    • ADP + PATP ~ +7.3kcal/mol

    • Energy from NADH can be used to drive synthesis of ATP several times.

      Important again to remember this is an oxidation-reduction reaction thus our friend Nerst is back:

      DeltaG'  =  -nFDE'


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Electron Transport is coupled to Oxidative Phosphorylation abbreviated enzymes are: IDH =

  • The idea of coupling is explained by Mitchell’s CHEMIOSMOTIC HYPOTHESIS

    • Basically coupling electron flow through the ETC to ATP synthesis

    • The Respiratory complexes are proton pumps. As electrons pass through complexes I, III, and IV, hydrogen ions are pumped across the inner mitochondrial membrane into the intermembrane space.

    • The proton concentration in the intermembrane space increases relative to the mitochondrial matrix

    • This generates a proton-motive force as a result of 2 factors: 1) Difference in pH and 2) Difference in electrical potential, delta si, between intermembrane space and the mitochondrial matrix.

    • ATP synthetase complex (complex V): Hydrogen ions pass back into the matrix through V, this drives ATP synthesis.

      • NADH 3ATP

      • FADH2 2 ATP: note bypass of Complex 1

        ATP synthesized in the matrix is transported out of the matrix via an ATP/ADP translocase (an antiport) also coupled to proton motive force.


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Inhibitors of Oxidative Phosphorylation abbreviated enzymes are: IDH =

Rotenone: e- transport inhibitor Complex I

  • Amytal: e- transport inhibitor Complex I

  • Antimycin: A e- transport inhibitor Complex III

  • Cyanide: e- transport inhibitor Complex IV

  • Carbon Monoxide: e- transport inhibitor Complex IV

  • Azide e- transport inhibitor Complex IV

  • 2,4,-dinitrophenol: Uncoupling agent transmembrane H+ carrier

  • Pentachlorophenol: Uncoupling agent transmembrane H+ carrier

  • Oligomycin: Inhibits ATP synthase

  • Thermogenin: also an uncoupler, component of brown fat

  • Malonate inhibits Complex II

  • There are others in your handout take a look at them.


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TCA cycle is regulated by the ratio of ADP, Pi/ ATP abbreviated enzymes are: IDH =

Under resting conditions, with a high cell energy charge, the demand for new synthesis of ATP is limited and, although the Proton Motive Force is high, flow of protons back into the mitochondria through ATP synthetase is minimal. When energy demands are increased, such as during vigorous muscle activity, cytosolic ADP rises and is exchanged with intramitochondrial ATP via the transmembrane adenine nucleotide carrier ADP/ATP translocase. Increased intramitochondrial concentrations of ADP cause the Proton Motive Force to become discharged as protons pour through ATP synthetase, regenerating the ATP pool.

The rate of electron transport is dependent on the PMF

ANY BLOCKADE AT ANY POINT IN THE ELECTRON TRANSPORT CHAIN STOPS ATP SYNTHESIS!!!!!!!!!

SOME MORE STUFF


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SAMPLE QUESTIONS abbreviated enzymes are: IDH =


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Choose the INCORRECT statement concerning the ATP-ADP cycle and the study of bioenergetics in the human body:

a. One half of the ATP-ADP cycle involves the coupling the energy derived from the hydrolysis of the high energy phosphate bonds of ATP to endergonic reactions so that they will occur spontaneously.

b. The work that requires energy derived from ATP hydrolysis includes the transport of electrons down the electron transport chain.

c. One half of the ATP-ADP cycle involves the generation of ATP that starts with the formation of reduced coenzymes like NADH and FADH2and the ultimate transfer of their electrons to oxygen

d. An important part of oxidative phosphorylation and ATP biosynthesis is the generation of an electrochemical gradient across the inner membrane of the mitochondria.

Many catabolic reactions, like the TCA cycle and fatty acid oxidation, provide the reduced coenzymes for the start of oxidative phosphorylation and ATP biosynthesis


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Since electron transport and oxidative phosphorylation are tightly coupled, which one of the following mechanisms BEST explains how ADP regulates the rate of electron transport during oxidative phosphorylation?

a. AMP concentrations are increased as ADP concentrations fall

b. Low [ADP] accelerates the Krebs (TCA) cycle reaction rates, thereby providing more NADH to activate electron transport

c. The transmembrane proton gradient is dissipated with low [ADP]

d. The ATP/ADP antiport system is not functional when mitochondrial [ADP] is low

e. Proton translocation across the inner mitochondrial membrane is decreased when ATP-synthase lacks bound ADP and Pi, secondarily retarding electron transport


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You isolate mitochondria from a group of patients that present with lactic acidosis and muscle weakness, and show that they are unable to: (1) oxidize reduced coenzyme Q, (2) translocate protons across their mitochondrial membranes to the intennembrane space against a concentration gradient with succinate added as the substrate, and (3) reduce cytochrome c. The biochemical defect in these patients most likely resides in their ... ?

A. Complex I (NADH dehydrogenase)

B. Complex II (succinate-Q reductase)

C. Complex III (cytochrome b-c1)

D. Complex IV (cytochrome oxidase)

E. Complex V (F1F0 ATPase)


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Which of the following orderings #1 present with lactic acidosis and muscle weakness, and show that they are - #5 of the various components of the electron transport chain and oxidative phosphorylation will effectively allow the development of an electrochemical potential sufficient to drive the generation of high energy phosphate bonds between ADP and Pi?

1. FMN, NADH dehydrogenase, ubiquinone, cytochrome c, cytochrome oxidase, F1F0-ATPase

2. Complex I, Complex III, ubiquinone, cytochrome a1-a3, cytochrome c, Complex IV, Complex V

3. FAD(2H)/succinate dehydrogenase, Coenzyme Q, cytochrome b-cl, cytochrome c, cytochrome a1-a3, F1F0-ATPase

4. NADH dehydrogenase, CoQ, cytochrome b-cl, cytochrome c, cytochrome oxidase, ATP synthase

5, NADH dehydrogenase, CoQ, cytochrome c, cytochrome oxidase, cytochrome b-cl, F1F0-ATPase

a. Both #1 and #2

b. Both #3 and #4

c. Only #4

d. Only #3

e. None of the above


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As a skilled cell biologist and biochemist, you cleverly devise a method for experimentally separating the F1 portion of ATP synthase from the membrane-bound Fo fragment in intact mitochondria. Which of the following metabolic effects do you observe?

a. Electron transport and oxygen consumption are inhibited

b. Electron transport and phosphorylation of ADP remain tightly coupled

c. The inner mitochondrial membrane remains impermeable to protons

d. Protons pass through the membrane-bound Fo fragment, but they do not sustain any ATP formation

e. The F1 fragment forms ATP at an accelerated rate until ADP is depleted or the proton gradient is dissipated


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Which of the following groups of enzymatic reactions, enzymes and substrates comprise important anaplerotic pathways for 4-carbon intermediates critical to the citric acid (TCA) cycle in the liver, muscle and nervous tissues?

a. conversion of pyruvate to acetyl CoA via pyruvate dehydrogenase and glutamate to a-ketoglutarate via transaminases

b. conversion of cc-ketoglutarate to glutamate and GABA

c. production of ketone bodies (acetoacetate and P-hydroxybutyrate)

d. conversion of pyruvate to oxaloacetate via pyruvate carboxylase, biotin, bicarbonate ion, and ATP

e. both (A) and (D)


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Regulation of tricarboxylic acid cycle activity in vivo may involve the concentration of all of the following EXCEPT:

acetyl CoA

ADP.

ATP.

CoA.

oxygen.


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NAD+ can be regenerated in the cytoplasm if NADH reacts with any of the following EXCEPT:

pyruvate.

dihydroxyacetone phosphate.

oxaloacetate.

the flavin bound to NADH dehydrogenase.

phosphoglycerate kinase.


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Glucokinase: any of the following EXCEPT:

has a Km considerably greater than the normal blood glucose concentration..

is found in muscle.

is inhibited by glucose 6-phosphate.

is also known as the GLUT-2 protein.

has glucose 6-phosphatase activity as well as kinase activity.


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A 7yr old female presents with anxiety, dizziness, sweating and nausea following brief periods of exercise. The symptoms are relieved by eating and do not occur if the patient is frequently fed small meals. Blood analysis indicates she is hypoglycemic following brief period of fasting, alanine fails to increase blood sugar, fructose or glycerol administration restores glucose to normal?

What Pathway is affected, which enzyme could it be? How would you confirm your speculation?


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  • After the BIOCHEM exam you and your friends decided to only drink “liquid-fire” (Bacardi 151) for the rest of the evening. The next morning you manage to wakeup with terrible ‘hangover’. Which of these molecules is most responsible for your hangover?

  • Lactic Acid

  • Pyruvate

  • Acetate

  • Acetyladehyde

  • Ethanol


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ADH drink “liquid-fire” (Bacardi 151) for the rest of the evening. The next morning you manage to wakeup with terrible ‘hangover’. Which of these molecules is most responsible for your hangover?alcohol dehydrogenase

AcDH acetyladehyde dehydrogenase

Acetaldehyde forms adducts with Proteins, nucleic acids, and other compounds results in hangover.

NADH/NAD+ imbalance causes Liver to over work.

Diversion of gluconeogenesis by Lactic Acid dehydrogenase decreases ability of Liver to deliver glucose to the blood.

In addition, there is increased synthesis of FAT. Acetate + CoA gives you acetyl-CoA which is a precursor for Fatty acid sythesis. You already have enough NADH to go to work. So let the FATTYLIVER BEGIN! HepatoMEGALLY! Lets go!


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Riboflavin Deficiency drink “liquid-fire” (Bacardi 151) for the rest of the evening. The next morning you manage to wakeup with terrible ‘hangover’. Which of these molecules is most responsible for your hangover?

FMN and FAD are both synthesized from riboflavin, which contains the electron-accepting ring structure of FAD

Severe Riboflavin deficiency decreases the ability of mitochondria to generate ATP via oxidative phosphorylation

In general, impairment of Complex I (NADH Dehydrogenase) induces formation of mitochondria with structural abnormalities.

Iron Deficiency Anemia

Characterized by decreased levels of Hb and other heme containing proteins in blood.

Iron-containing cytochromes and Fe-S centers of ETC are decreased as well.

Fatigue partly due to impaired ETC for ATP generation

ETC inhibitors at specific sites

Rotenone and Amytal block Complex I

Antimycin blocks cytochrome b1 in Complex III

Cyanide blocks cytochrome a/a3 in Complex IV. Prevents reduction of e- from reduced cytochrome c.

CO binds to reduced iron of cytochrome oxidase

Cyanide Poisoning

CN- causes a rapid and extensive inhibition of ETC at the cytochrome oxidase step. Prevents O2 from serving as the final e- acceptor.

Mitochondrial respiration and energy production cease, resulting in cell death

Occurs from tissue asphyxiation, most notably in the Nervous System

Treatment: nitrites administered to convert oxyHb to MetheHb, which can then compete with cytochrome a,a3 for the CN-, forming a complex.

CLINICAL CORRELATIONS


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Oxidative Phosphorylation II “the uncoupling of ETC and Ox-Phos”

Uncoupling of ETC with Ox-Phos

Proton gradient from ETC coupled to ATP production from Oxidative Phosphorylation. If uncoupled and proton gradient dissipated, ATP and ADP concentrations lose their ability to regulate the rate of e- transport.

Uncouplers:proton ionophores, which rapidly transport H+ from cytosolic to matrix side of inner mitochondiral membrane

DNP – picks up H+ on cyto side, drops H+ on matrix side

Oligomycin: inhibits F1F0-ATPase…ATP synthesis stops.

Respiration and transport are blocked

Addition of an uncoupler (DNP) induces initiation of O2 consumption…ETC continues but w/o ATP synthesis since the pathways are uncoupled.

Brown Adipose Tissue and Thermogenesis

Large deposits of brown fat around vital organs (in human infants)…specialized for ‘non-shivering thermogenesis.’

Cold or excessive food intake stimulates NE release

Then Thermogenin, proton conductance uncoupler, is activated, pumping H+ back into mitochondria…dissipating the gradient.

ETC is induced, increasing rate of NADH and FADH2 oxidation, which generates more heat = biological heating pad


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Hyperthyroidism – Grave’s Disease Ox-Phos”

Thyroid hormone influences bioenergetics via actions on mitochondrial ox phos.

In Hyperthyroidism, energy derived from ox. Phos is significantly less than normal.

Thryoid causes ‘uncoupling’ of Ox Phos.

Results in increased heat production – patients complain of feeling hot and sweaty.

Salicylate (aspirin) poisoning

At high concentrations, salicylate can partially uncouple mitochondrial Ox Phos.

Decreased ATP [ ] and increased cytosolic AMP induce glycolysis

Results in increased blood pyruvate and lactate and metabolic acidosis and fever

Myoclonic Epileptic Ragged Red Fiber Disease (MERRF)

Debilitating, progressive spontaneous muscle jerking

Mitochondrial myopathy with enlarged, abnormal mitochondria

Neurosensory hearing loss, dementia, hypoventilation, mild cardiomyopathy

Maternal inheritance (sex linked)

Impaired energy metabolism….lactic acidosis


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Pentose Phosphate Pathway Ox-Phos”

Hemolysis caused by Reactive Oxygen Species (ROS)

G6PD deficiency in pentose phosphate pathway

Causes increased production of radicals from GSH, since can’t produce sufficient NADPH to re-reduce glutathione….result in hemolysis

Heinz Bodies in RBCs

Due to G6PD deficiency

RBCs need the enzyme to re-reduce glutathione with NADPH to protect against oxidative stress

ROS peroxidation of membrane lipids lyses the RBC membrane

G6PD Mediterranean disease most severe G6PD deficiency

Lecture 21 – Monosaccharides and interconversion of sugars

Classical Galactosemia

Deficiency of Galactosyl-1-P uridylyltransferase

Accumulation of G-1-P in tissues and inhibition of glycogen metabolism, which require UDP-sugars

Higher level of galactose in blood and urine

More serious form

Non-Classical Galactosemia

Galactokinase deficiency

Unable to convert galactose to galactose-1-P


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Glycogen Synthesis Ox-Phos”

Glucose Toxicity

Dysfunction of glycogen synthase

Due to hyperglycemia…produces insulin resistance

Due to production of hexosamines that inhibit hexokinase, protein phosphatase 1, and glycogen synthase.

Lecture 23 – Glycogen Degradation

Von Gierke’s Disease

Defective G-6-Phosphatase enzyme

Increased amount of glycogen, normal structure

Affects liver and kidney

Massive enlargement of the liver. Severe hypoglycemia, ketosis, hyperuricemia, hyperlipemia.

Lecture 24 – Glucose/Glycogen Regulation

Type I – Insulin-dependent diabetes mellitus

Hyperglycemic

Continuous glucagon expression causes ketogenesis, lipolysis, and gluconeogenesis.

Hyperchylomicronemia occurs (liver TG syn and VLDL transport faster than adipose LPL breakdown of TG)

Risk of ketoacidosis

Type II – Noninsulin-dependent Diabetes Mellitus

Hyperglycemic

Peripheral tissues insulin resistant

Glucose accumulates in blood due to poor uptake by peripheral tissues, particularly muscles

Hypertriacylglycerolemia, which results from increase of VLDL without hyperchylomicronemia. New FA and VLDL synthesized in liver instead of increased delivery of fatty acids from adipose tissue.


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Pentose Phosphate Pathway Ox-Phos”

What is the PPP and why is it important?


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Pentose Phosphate Pathway Ox-Phos”

What is the PPP and why is it important?

The pentose phosphate pathway is primarily an anabolic pathway that utilizes the 6 carbons of glucose to generate 5 carbon sugars and reducing equivalents


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Pentose Phosphate Pathway Ox-Phos”

  • To generate reducing equivalents, in the form of NADPH, for reductive biosynthesis reactions within cells

  • To provide the cell with ribose-5-phosphate (R5P) for the synthesis of the nucleotides and nucleic acids

  • Although not a significant function of the PPP, it can operate to metabolize dietary pentose sugars derived from the digestion of nucleic acids as well as to rearrange the carbon skeletons of dietary carbohydrates into glycolytic/gluconeogenic intermediates


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Pentose Phosphate Pathway Ox-Phos”

  • The reactions of fatty acid biosynthesis and steroid biosynthesis utilize large amounts of NADPH. As a consequence, cells of the liver, adipose tissue, adrenal cortex, testis and lactating mammary gland have high levels of the PPP enzymes.

  • Erythrocytes utilize the reactions of the PPP to generate large amounts of NADPH used in the reduction of glutathione

  • The conversion of ribonucleotides to deoxyribonucleotides (through the action of ribonucleotide reductase) requires NADPH as the electron source, therefore, any rapidly proliferating cell needs large quantities of NADPH


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Oxidative Pathway Ox-Phos”

Pentose Phosphate Pathway

The reactions of the PPP operate exclusively in the cytoplasm. From this perspective it is understandable that fatty acid synthesis (as opposed to oxidation) takes place in the cytoplasm

The oxidation steps, utilizing glucose-6-phosphate (G6P) as the substrate, occur at the beginning of the pathway and are the reactions that generate NADPH

Reactions catalyzed by glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase generate one mole of NADPH each for every mole of glucose-6-phosphate (G6P) that enters the PPP


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Non-oxidative Pathway Ox-Phos”

Pentose Phosphate Pathway

Non-oxidative reactions are to convert dietary 5 carbon sugars into both 6 (fructose-6-phosphate) and 3 (glyceraldehyde-3-phosphate) carbon sugars which can then be utilized by the pathways of glycolysis

The primary enzymes involved in the non-oxidative steps of the PPP are transaldolase and transketolase

Transketolase functions to transfer 2 carbon groups from substrates of the PPP, thus rearranging the carbon atoms that enter this pathway. Like other enzymes that transfer 2 carbon groups, transketolase requires thiamine pyrophosphate (TPP) as a co-factor in the transfer reaction

Transaldolase transfers 3 carbon groups and thus is also involved in a rearrangement of the carbon skeletons of the substrates of the PPP. The transaldolase reaction involves Schiff base formation between the substrate and a lysine residue in the enzyme


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What’s the point? Ox-Phos”

Pentose Phosphate Pathway


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What’s the point? Ox-Phos”

Pentose Phosphate Pathway

R5P production

Oxidation of G6P, a 6 carbon sugar, into a 5 carbon sugar

Generation of NADPH

3 carbon sugar generated is glyceraldehyde-3-phsphate which can be shunted to glycolysis and oxidized to pyruvate OR it can be utilized by the gluconeogenic enzymes to generate more 6 carbon sugars (fructose-6-phosphate or glucose-6-phosphate)


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RBCs and the PPP Ox-Phos”

Pentose Phosphate Pathway

Predominant pathways of carbohydrate metabolism in the red blood cell (RBC) are glycolysis, the PPP and 2,3-bisphosphogylcerate (2,3-BPG)

Glycolysis provides ATP for membrane ion pumps and NADH for re-oxidation of methemoglobin

The PPP supplies the RBC with NADPH to maintain the reduced state of glutathione (Glutathione can reduce disulfides nonenzymatically)

Oxidative stress generates peroxides that in turn can be reduced by glutathione to generate water

Inability to maintain reduced glutathione in RBCs leads to increased accumulation of peroxides, predominantly H2O2, that in turn results in a weakening of the cell wall and concomitant hemolysis

Glutathione removes peroxides via the action of glutathione peroxidase. The PPP in erythrocytes is essentially the only pathway for these cells to produce NADPH



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Glycogen Ox-Phos” is a polymer of glucose residues linked by

  • a(14) glycosidic bonds, mainly

  • a(16) glycosidic bonds, at branch points

    Glycogen chains & branches are longer than shown

    Glucose is stored as glycogen predominantly in liver and muscle cells.


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Glycogen catabolism Ox-Phos” (breakdown):

Glycogen Phosphorylase catalyzes phosphorolytic cleavage of the a(14) glycosidic linkages of glycogen, releasing glucose-1-phosphate as reaction product.

glycogen(n residues) + Pi

glycogen (n–1 residues) + glucose-1-phosphate


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Commonly used terminology: Ox-Phos”

  • "a" is the form of the enzyme that tends to be active, and independent of allosteric regulators (in the case of Glycogen Phosphorylase, when phosphorylated).

  • "b" is the form of the enzyme that is dependent on local allosteric controls (in the case of Glycogen Phosphorylase when dephosphorylated).


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Glycogen catabolism Ox-Phos”

Most people don’t know…

The relative activity of the un-modified phosphorylase enzyme (phosphorylase-b) is sufficient to generate enough glucose-1-phosphate for entry into glycolysis for the production of sufficient ATP to maintain the normal resting activity of the cell; This is true in both liver and muscle cells


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Glycogen Phosphorylase Ox-Phos” in muscle is subject to allosteric regulation by AMP, ATP, and glucose-6-phosphate. A separate isozyme of Phosphorylase expressed in liver is less sensitive to these allosteric controls.

  • AMP (present significantly when ATP is depleted) activates Phosphorylase, promoting the relaxed conformation.

  • ATP& glucose-6-phosphate, which both have binding sites that overlap that of AMP, inhibit Phosphorylase,promoting the tense conformation.

  • Thus glycogen breakdown is inhibited when ATP and glucose-6-phosphate are plentiful.


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Regulation by covalent modification Ox-Phos” (phosphorylation):

The hormones glucagonandepinephrine activate G-protein coupled receptors to trigger cAMP cascades.

  • Both hormones are produced in response to low blood sugar.

  • Glucagon, which is synthesized by a-cells of the pancreas, activates cAMP formation in liver.

  • Epinephrine activates cAMP formation in muscle.


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Glycogen catabolism Ox-Phos”

  • In response to lowered blood glucose the a cells of the pancreas secrete glucagon which binds to cell surface receptors on liver and several other cells; Liver cells are the primary target for the action of this peptide hormone

  • Activation of the enzyme adenylate cyclase which leads to a large increase in the formation of cAMP

  • cAMP binds to an enzyme called cAMP-dependent protein kinase, PKA. This leads to PKA-mediated phosphorylation of phosphorylase kinasePhosphorylase kinase activates the enzyme which in turn phosphorylates the b form of phosphorylase

  • Phosphorylation of phosphorylase-b greatly enhances its activity towards glycogen breakdown (phosphorylase-a)

  • The net result is an extremely large induction of glycogen breakdown in response to glucagon binding to cell surface receptors



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The cAMP cascade results in activated.phosphorylation of a serine hydroxyl of Glycogen Phosphorylase, which promotes transition to the active (relaxed) state.

The phosphorylated enzyme is less sensitive to allosteric inhibitors.

Thus, even if cellular ATP & glucose-6-phosphate are high, Phosphorylase will be active.

The glucose-1-phosphate produced from glycogen in liver may be converted to free glucose for release to the blood.

With this hormone-activated regulation, the needs of the organism take precedence over needs of the cell.


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Glycogen catabolism activated.

This identical cascade of events occurs in skeletal muscle cells

However, in these cells the induction of the cascade is the result of epinephrine binding to receptors on the surface of muscle cells

(Ca2+ ion-mediated pathway to phosphorylase kinase activation is through activation of a-adrenergic receptors by epinephrine)

Epinephrine is released from the adrenal glands in response to neural signals indicating an immediate need for enhanced glucose utilization in muscle, the so called fight or flight response

Muscle cells lack glucagon receptors. The presence of glucagon receptors on muscle cells would be futile anyway since the role of glucagon release is to increase blood glucose concentrations and muscle glycogen stores cannot contribute to blood glucose levels…why?


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Glycogen catabolism activated.

  • Regulation of phosphorylase kinase activity is also affected by two distinct mechanisms involving Ca2+ ions

  • The ability of Ca2+ ions to regulate phosphorylase kinase is through the ubiquitous protein, calmodulin

  • Calmodulin is a calcium binding protein; binding induces a conformational change in calmodulin which in turn enhances the catalytic activity of the phosphorylase kinase towards its substrate, phosphorylase-b.

  • This activity is crucial to the enhancement of glycogenolysis in muscle cells where muscle contraction is induced via acetylcholine stimulation at the neuromuscular junction

  • The effect of acetylcholine release from nerve terminals at a neuromuscular junction is to depolarize the muscle cell leading to increased release of sarcoplasmic reticulum stored Ca2+, thereby activating phosphorylase kinase

  • Thus, not only does the increased intracellular calcium increase the rate of muscle contraction it increases glycogenolysis which provides the muscle cell with the increased ATP it also needs for contraction


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Phosphorylase Kinase activated. in muscle includes calmodulin as its d subunit. Phosphorylase Kinase is partly activated by binding of Ca++ to this subunit

Phosphorylation of the enzyme, via a cAMP cascade induced by epinephrine, results in further activation

These regulatory processes ensure release of phosphorylated glucose from glycogen, for entry into Glycolysis to provide ATP needed for muscle contraction.


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Pyridoxal phosphate activated.(PLP), a derivative of vitamin B6, serves as prosthetic group for Glycogen Phosphorylase.


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A class of drugs developed for treating the hyperglycemia of diabetes (chloroindole-carboxamides), inhibit liver Phosphorylase allosterically.

These inhibitors bind at the dimer interface, stabilizing the inactive (tense) conformation.

Question: Why would an inhibitor of Glycogen Phosphorylase be a suitable treatment for diabetes?


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Debranching enzyme diabetes (chloroindole-carboxamides), inhibit liver Phosphorylase allosterically. has 2 independent active sites, consisting of residues in different segments of a single polypeptide chain:

  • The transferase of the debranching enzyme transfers 3 glucose residues from a 4-residue limit branch to the end of another branch, diminishing the limit branch to a single glucose residue

  • The a(16) glucosidase moiety of the debranching enzyme then catalyzes hydrolysis of the a(16) linkage, yielding free glucose. This is a minor fraction of glucose released from glycogen

  • The major product of glycogen breakdown is glucose-1-phosphate, from Phosphorylase activity.


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Phosphoglucomutase diabetes (chloroindole-carboxamides), inhibit liver Phosphorylase allosterically. catalyzes this reversible reaction


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The product diabetes (chloroindole-carboxamides), inhibit liver Phosphorylase allosterically.glucose-6-phosphate may enter Glycolysis or (in liver) be dephosphorylated for release to the blood

Liver Glucose-6-phosphatase catalyzes the following, essential to the liver's role in maintaining blood glucose:

glucose-6-phosphate + H2O glucose + Pi

Most other tissues lack this enzyme…why??


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Glycogen synthesis diabetes (chloroindole-carboxamides), inhibit liver Phosphorylase allosterically.

Uridine diphosphate glucose (UDP-glucose) is the immediate precursor for glycogen synthesis

As glucose residues are added to glycogen, UDP-glucose is the substrate and UDP is released as a reaction product.


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UDP-glucose diabetes (chloroindole-carboxamides), inhibit liver Phosphorylase allosterically.is formed from glucose-1-phosphate:

  • glucose-1-phosphate + UTPUDP-glucose + PPi

  • PPi + H2O 2 Pi

    Overall:

  • glucose-1-phosphate + UTPUDP-glucose + 2 Pi

    Spontaneous hydrolysis of the ~P bond in PPi (P~P) drives the overall reaction

    Cleavage of PPi is the only energy cost for glycogen synthesis (one ~P bond per glucose residue).

    Glycogenin initiates glycogen synthesis.

    Glycogenin is an enzyme that catalyzes glycosylation of one of its own tyrosine residues.


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A glycosidic bond is formed between the anomeric C1 of the glucose moiety derived from UDP-glucose and the hydroxyl oxygen of a tyrosine side-chain of Glycogenin.

UDP is released as a product.


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Glycosylation at C4 of the O-linked glucose product yields an O-linked disaccharide with a(1®4) glycosidic linkage. UDP-glucose is again the glucose donor

This is repeated until a short linear glucose polymer with a(1®4) glycosidic linkages is built up on Glycogenin


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Glycogen Synthase an O-linked disaccharide with catalyzes transfer of the glucose moiety of UDP-glucose to the hydroxyl at C4 of the terminal residue of a glycogen chain to form an a(1® 4) glycosidic linkage:

glycogen(n residues) + UDP-glucose

glycogen(n +1 residues) + UDP

A separate branching enzyme transfers a segment from the end of a glycogen chain to the C6 hydroxyl of a glucose residue of glycogen to yield a branch with an a(1®6) linkage.


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Both synthesis an O-linked disaccharide with &breakdownofglycogen are spontaneous

If both pathways were active simultaneously in a cell, there would be a "futile cycle" with cleavage of one ~P bond per cycle (in forming UDP-glucose)

To prevent such a futile cycle, Glycogen Synthase and Glycogen Phosphorylase are reciprocally regulated, by allosteric effectors and by phosphorylation.


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Glycogen Synthase an O-linked disaccharide with is allostericallyactivatedby glucose-6-P (opposite of effect on Phosphorylase)

Thus Glycogen Synthase is active when high blood glucose leads to elevated intracellular glucose-6-P

It is useful to a cell to store glucose as glycogen when the input to Glycolysis (glucose-6-P), and the main product of Glycolysis (ATP), are adequate.


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High cytosolic glucose-6-phosphate, which would result when blood glucose is high, turns off the signal with regard to glycogen synthesis

The conformation of Glycogen Synthase induced by the allosteric activator glucose-6-phosphate is susceptible to dephosphorylation by Protein Phosphatase (PP1)


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The blood glucose is high, turns off the signal with regard to glycogen synthesiscAMP cascade induced in liver by glucagon or epinephrine has the opposite effect on glycogen synthesis.

Glycogen Synthase is phosphorylated by Protein Kinase A as well as by Phosphorylase Kinase.

Phosphorylation of Glycogen Synthase promotes the "b" (less active) conformation.

The cAMP cascade thus inhibits glycogen synthesis.

Instead of being converted to glycogen, glucose-1-P in liver may be converted to glucose-6-P, and dephosphorylated for release to the blood.


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Insulin blood glucose is high, turns off the signal with regard to glycogen synthesis, produced in response to high blood glucose, triggers a separate signal cascade that leads to activation of Phosphoprotein Phosphatase

This phosphatase catalyzes removal of regulatory phosphate residues from Phosphorylase, Phosphorylase Kinase, & Glycogen Synthase enzymes

Thus insulin antagonizes effects of the cAMP cascade induced by glucagon & epinephrine


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Glycogen blood glucose is high, turns off the signal with regard to glycogen synthesisStorage Diseasesare genetic enzyme deficiencies associated with excessive glycogen accumulation within cells

Some enzymes whose deficiency leads to glycogen accumulation are part of the inter-connected pathways shown here


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  • When an enzyme defect affects mainly glycogen storage in blood glucose is high, turns off the signal with regard to glycogen synthesisliver, a common symptom is hypoglycemia, relating to impaired mobilization of glucose for release to the blood during fasting.

  • When the defect is in muscle tissue, weakness & difficulty with exercise result from inability to increase glucose entry into Glycolysis during exercise.

  • Additional symptoms depend on the particular enzyme that is deficient. 


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Summary blood glucose is high, turns off the signal with regard to glycogen synthesis


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