bacterial ecology in arthropod digestive tracts
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Bacterial Ecology in Arthropod Digestive Tracts

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Bacterial Ecology in Arthropod Digestive Tracts Casey Eisele Significance of Research Arthropods aid in ruminant digestion Little research has been conducted using arthropods Studies suggest that arthropods benefit from both enzymatic activity (cellulose degradation)

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Presentation Transcript
significance of research
Significance of Research
  • Arthropods aid in ruminant digestion
  • Little research has been conducted using arthropods
  • Studies suggest that arthropods benefit from both
    • enzymatic activity (cellulose degradation)
    • products of fermentation
within the guts of arthropods
Within the guts of Arthropods
  • Microbes have been isolated from cockroaches, crickets, mealworms, desert millipedes, and scarab beetles
  • Normal flora
    • high ratio of microbes/biomass
    • attachment to endothelium
      • cockroaches: 100 species
      • crickets: 25 species
materials and methods
Materials and Methods
  • Arthropods were sacrificed, sterilized and dissected
  • Bacteria was liberated from the lining of the gut using a dilute solution of EDTA and sonication
  • 1/10,000 dilutions were plated on
    • T Soy Agar
  • Isolated bacteria were used to inoculate
    • Phenol Red Broth
    • Lactate Broth
    • Cellulose plates and broth
results
Results
  • Bacteria were isolated from all three arthropod species
t soy agar periplaneta americana
T Soy Agar (Periplaneta americana)
  • Isolated 5 species
    • Underestimation
    • Psychotolerant

Careful, they’re quick.

t soy agar zophobas morio
T Soy Agar (Zophobas morio)
  • Isolated 2 species
populations and diversity
Populations and Diversity

Bacteria/Biomass

  • Weight of gut # of species
    • Cricket = 2mg 2
    • Mealworm =54mg 2
    • Cockroach =25mg >5
  • Bacteria/Biomass ratios confirmed the findings of Cazemier et al. and other studies

Bacterial Count

(1x10 ^5 cfu/mg)

Mealworm

Species

biochemical tests
1) Fermentation

Glycolytic Pathway

host/bacteriabacteria

Lactose (6 C) — —> Lactate (3 C) —> Pyruvate,Acetic Acid, Ethanol

Phenol red broth Lactate Broth

Biochemical Tests
results11
Results
  • Bacteria were isolated from all three arthropod species
  • 6 species fermented lactose
glycolytic pathway
Lactose —> Lactate —> Pyruvate/Acetic/Ethanol

Cockroach

Species 1 no

Species 2 proteolytic

Species 3

Species 4

Species 5

Mealworm

Species 6

Species 7

Cricket

Species 8

Species 9 proteolytic

Glycolytic Pathway
lactose fermentation
Lactose Fermentation

lactose fermenters

control

proteolytic

proteolytic

cricket

mealworm

cockroach

results14
Results
  • Bacteria were isolated from all three arthropod species
  • 6 species fermented lactose
  • All Bacteria reduced lactate
glycolytic pathway15
Glycolytic Pathway

Lactose —> Lactate —> Pyruvate/Acetic/Ethanol

  • Cockroach
    • Species 1 no
    • Species 2 proteolytic
    • Species 3
    • Species 4
    • Species 5
  • Mealworm
    • Species 6
    • Species 7
  • Cricket
    • Species 8
    • Species 9 proteolytic
biochemical tests16
Biochemical Tests
  • 1) Fermentation
  • 2) Cellulose Degradation

bacteria

Cellulose —> Simple Sugars (6 C)

Cellulose plate/broth

cellulose degradation
Cellulose Degradation
  • Cellulose tests

were inconclusive

conclusions
Conclusions
  • My study confirmed the presence of large bacterial populations living within the gut of arthropods
  • These bacteria exhibited the ability to ferment compounds, whose end-products may be used by the host for nutrition
  • Further testing would be needed to determine cellulose-degrading capabilities
acknowledgements
Acknowledgements
  • Thank You
    • Professor Rose
    • Professor Smith
    • Tony
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