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Carbohydrates

Carbohydrates. What are they? Formula = (CH 2 O) n where n > 3 Also called sugar Major biomolecule in body What do cells do with carbs? Oxidize them for energy Store them to oxidize later for energy Use them as components of larger molecules Sugars attached to proteins and lipids

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Carbohydrates

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  1. Carbohydrates What are they? Formula = (CH2O)n where n > 3 Also called sugar Major biomolecule in body What do cells do with carbs? Oxidize them for energy Store them to oxidize later for energy Use them as components of larger molecules Sugars attached to proteins and lipids Where do carbs come from? 1. Plants “fix” CO2 from air to synthesize sugars 6CO2 + 6H2O ---> C6H12O6 + 6O2 2. Other organisms eat sugar or make sugar from smaller organic compounds

  2. Carbohydrates What carbs are found in cells? 1. Monosaccharides single sugar 2. Oligosaccharides (disaccharides most abundant) glycosidic bonds often linked to proteins - glycoproteins or to lipids - glycolipids 3. Polysaccharides large polymers structural - cellulose (linear) storage - starch (branched) Glc Glc Glc Maltose, a disaccharide Glc Glc n -Amylose, component of starch

  3. Monosaccharides An aldehyde or ketone with > 1 hydroxyl group Colorless, crystalline, usually sweet One carbonyl group aldose ketose

  4. Monosaccharides Aldoses Most common in nature - hexoses (6-carbon sugars) Components of nucleotides and nucleic acids Six carbons D isomers Five carbons D isomers

  5. Monosaccharides Other Aldoses

  6. Monosaccharides Ketoses

  7. Monosaccharides Some exceptions to rule on D isomers only L-Arabinose and sugars in glycoconjugates

  8. Monosaccharides Epimers - Two sugars that differ only in configuration around one carbon atom

  9. Monosaccharides Why are these particular sugars utilized? Evolution Glucose forms especially stable structures Monosaccharides can spontaneously cyclize C1 not chiral in open chain form, but it is in pyranose Stereoisomers differing only at C1 are called  and  anomers C1 is anomeric carbon - carbon that used to be the carbonyl C

  10. Monosaccharides Monosaccharides

  11. Monosaccharides Sugars can be reducing agents Fehling’s reaction Cuprous ion (Cu+) forms red precipitate Measure ppt. - estimate [glc] reduction oxidization Measure blood glc by measuring amount of H2O2

  12. Glucose meters Captain glucose and meter boy

  13. Oligosaccharides & Polysaccharides Glycosidic bonds Hydroxyl on anomeric carbon is reactive and can condense with an alcohol to form a glycosidic bond Reducing end (hemiacetal) - still has free anomeric carbon Nonreducing end - no anomeric carbon

  14. Disaccharides Occurs naturally only in milk Table sugar Formed by plants, not us No anomeric carbon Glycosidases hydrolyze glycosidic bonds, specific for particular linkages Lactose intolerance: lack lactase, the enzyme that can break down lactose

  15. Polysaccharides Homopolysaccharides - starch, glycogen (storage); cellulose, chitin (structure) Heteropolysaccharides - bacterial cell membrane, extracellular space in animal cells

  16. Polysaccharides - Storage 2 types of glc polymers Amylose - linear, 14 Amylopectin - highly branched, 14 glycosidic, 16 branch points

  17. Polysaccharides - Storage Starch - 14 glycosidic, 16 branch points; 1 branch/24-30 residues Glycogen - 14 glycosidic, 16 branch points, more extensively branched: 1 branch/8-12 residues nonreducing ends - glc can be mobilized quickly Lots in liver, some in muscle Liver stores glycogen equivalent to glc concentration of 400 mM (blood glc ~5 mM)

  18. Polysaccharides - - Structure Cellulose fibrous, tough, water insoluble, plant cell wall, humans can’t digest linear, unbranched, lots of glc linked by (14) glycosidic linkages Chitin tough, water insoluble, component of hard exoskeleton (insect, lobster, crab), indigestible by humans linear, uses modified sugars (N-acetyl-D-glucosamine) with (14) linkages

  19. Polysaccharides- structure Starch - floppy left-handed helix Cellulose - rigid chain

  20. Polysaccharides Glycogen, starch ingested in diet hydrolyzed by -amylases (in saliva and intestinal secretions) that break 14 linkages Most animals cannot use cellulose as fuel because no enzyme to hydrolyze 14 linkages Termites readily digest cellulose (wood) because their intestinal tract harbors microorganism that secretes cellulase (breaks 14 linkages) Only vertebrates able to use cellulose as food are cattle and other ruminants (sheep, goats, camel, giraffes) that have extra stomach compartment (rumen) that has bacteria/protists that secrete cellulase

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