1 / 21

Epifluorescence

Confocal. Epifluorescence. Zoom 1. Zoom 2. Zoom 4. x - y. x - z. 3D Deconvolution. Sassoè-Pognetto et al., J Comp Neurol 2000. Point Spread Function. Disco di Airy. Sassoè-Pognetto et al., J Comp Neurol 2000. FITC-TRITC emission. VGAT / Calb. WT. GABA A R a 1 KO.

orsin
Download Presentation

Epifluorescence

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Confocal Epifluorescence

  2. Zoom 1 Zoom 2 Zoom 4

  3. x - y x - z 3D Deconvolution Sassoè-Pognetto et al., J Comp Neurol 2000 Point Spread Function Disco di Airy

  4. Sassoè-Pognetto et al., J Comp Neurol 2000

  5. FITC-TRITC emission

  6. VGAT/Calb WT GABAARa1 KO Wulff et al., Nat Neurosci in press

  7. a10/0 GABA parallel fiber GABA-positive terminals in the molecular layer make asymmetric synapses with Purkinje cell spines Fritschy et al., J Neurosci 2006

  8. NL2/Car8/GAD GABAAa10/0 sp sp sp NL2/mGluR1a/GAD sp NL2 does not localize ectopically at heterologous synapses with PC spines GABA Patrizi et al., PNAS 2008

  9. Schneider Gasser et al et al.,

  10. Two-photon microscopy Two photons of low energy (infrared range) can excite a fluorophore → emission of a fluorescence photon at a higher energy Excitation is restricted to a tiny focal volume (rejection of out-of-focus signals) Longer wavelengths penetrate deeper into tissue (1 mm) Lower energy photons cause relatively low damage outside the focal volume → in vivo imaging

More Related