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CSHL Computational Cell Biology Islet Assessment

CSHL Computational Cell Biology Islet Assessment. July 2012 Allan J. Ropski University of Illinois at Chicago Department of Surgery. Islets of Langerhans. Islet Potency Index FDA requirement for use as a biological drug Sterility, identity, and purity are established

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CSHL Computational Cell Biology Islet Assessment

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  1. CSHL Computational Cell BiologyIslet Assessment July 2012 Allan J. Ropski University of Illinois at Chicago Department of Surgery

  2. Islets of Langerhans • Islet Potency Index • FDA requirement for use as a biological drug • Sterility, identity, and purity are established • Reliably predict transplant outcomes • Glucose-stimulated insulin secretion; β-cell specific • In-vivo streptozotocin treated mice; Potency Index • UIC-MS islet assays • Phase III human clinical trials*; 52-week follow-up • Microfluidic optimization • Ca2+; Mitochondrial Potential; Insulin release • High-throughput assays

  3. Human Islet Cell Composition • 6 isolations • 32 sampled islets • Ranges α-cells 28 – 75% β-cells 10 – 65% δ-cells 1 – 22% Assessment of Human Pancreatic Islet Architecture and Composition by Laser Scanning Confocal Microscopy Journal of Histochemistry & Cytochemistry; Volume 53(9): 1087–1097, 2005 Marcela Brissova, Michael J. Fowler, Wendell E. Nicholson, Anita Chu, Boaz Hirshberg, David M. Harlan, and Alvin C. Powers

  4. Microfluidic System

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