1. Title Subtitle
3. Neonatal complications of DM
4. Monitoring glycaemic control in DM
5. Potential to measure glycated fetal Hb Roberts et al (2001) evaluated ESI-MS for the analysis of HbA1c
Technique enables assessment of glycation of ? and ? chains of Hb and any other Hb variant
? Potential to measure glycation and acetylation of HbF
6. Aim of study
To assess the relationship between glycated and acetylated haemoglobin in the neonate and glycated haemoglobin in the mother
7. Methods 1 Quattro Ultima mass spectrometer (Waters, Ltd)
10 ?L aliquot of whole blood (F EDTA) was diluted in
490 ?L of deionised water
20 ?L aliquot of above diluted with:
40 ?L of 10 mL/L formic acid
40 ?L of deionised water
100 ?L of acetonitrile
Samples desalted with AG 50-X8 resin (BioRad Laboratories).
8. Methods 2 HbA1c analysis - Menarini-Arkay
HA-8160 HPIEC system (Menarini Diagnostics)
Glucose analysis - Roche D modular unit
glucose oxidase method (Roche Diagnostics)
Statistical analysis of data done using Astute (Microsoft Excel® [version 5])
9. Samples used in study
12. Calculating the level of GHb and acetylated Haemoglobin GHb (%) = 50([?g/(?+?g)] + [*g/(*+*g)])
* = ? or ? chain intensity
Acetylated HbF (%) = 100[?a/? + ?a]
13. Is there a relationship between GHb in the mother and neonate?
14. Mean level (? 1 SD) of glucose and GHb observed in diabetic and non diabetic pregnant women and their offspring.
15. Mean level (? 1 SD) of glucose and GHb observed in diabetic and non diabetic pregnant women and their offspring.
16. Mean level (? 1 SD) of glucose and GHb observed in diabetic and non diabetic pregnant women and their offspring.
17. Mean level (? 1 SD) of glucose and GHb observed in diabetic and non diabetic pregnant women and their offspring.
18. Distribution of GHb (%) observed in non diabetic and diabetic women and their offspring
20. Distribution of acetylated Hb levels (%) in neonates born to non diabetic and diabetic women
21. Conclusions Preliminary data suggest that there may be a significant correlation between maternal and neonatal GHb levels in the diabetic group.
Absence of a significant difference between maternal GHb levels suggests good long term glycaemic control in diabetic individuals during pregnancy
22. Future work Collect more samples from diabetic women and their respective neonate to allow a more reliable assessment of the relationship between GHb levels in the mother and neonate
23. Acknowledgements Norman Roberts (Consultant Biochemist, RLBUH)
Anthony Stott (Consultant Biochemist, RLBUH)
Steve Walkinshaw (Consultant Obstetrician, LWH)
Lorna Wood (Diabetes Nurse Specialist, LWH)
Brian Green (Researcher, Waters)