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AFFYMETRIX GENECHIP ARRAY STATION. By. Pierce Chuang, Vishu Rajamani, Jeremy Rasiah. Overview. Introduction to DNA/RNA Sequencing Techniques Affymetrix GeneChip Station GeneChip Station Architecture Manufacturing Process Questions. Introduction to DNA/RNA. Introduction DNA/RNA (cont’d).

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affymetrix genechip array station

AFFYMETRIX GENECHIP ARRAY STATION

By

Pierce Chuang, Vishu Rajamani, Jeremy Rasiah

overview
Overview
  • Introduction to DNA/RNA
  • Sequencing Techniques
  • Affymetrix GeneChip Station
  • GeneChip Station Architecture
  • Manufacturing Process
  • Questions
dna sequencing techniques
DNA Sequencing Techniques
  • Chain-terminator method (Sanger)
    • Dideoxynuclotides triphosphates (ddNTPs) as DNA chain terminators
    • Sequence roughly 550,000 bases in a day
    • Requires thirty years of uninterrupted operation to sequence all nucleotides in the human genome
dna sequencing techniques1
DNA Sequencing Techniques
  • Sequencing By Hybridization (SBH)
    • Ex, target DNA sequence, AGCCTAGCTGAA

Affymetrix Microarray Specification

genechip array
GeneChip Array

source: http://bach2.biosci.arizona.edu/

results
Results

source: http://www.med.upenn.edu/

detector architecture
Detector Architecture

source: http://www.affymetrix.com/

genechip array1
GeneChip Array

source: http://bach2.biosci.arizona.edu/

array manufacturing process
Array Manufacturing Process
  • 2: substrates, SiO2, Si, GaAs, or Glass.
  • 6: Linker Molecules. Ex, Aryl Acetylene
  • 8: Mask, for photolithographic process
  • 10: Activated Linker Molecules
array manufacturing process2
Array Manufacturing Process
  • This process is repeated several times to produce longer probes.
  • Full length probes are usually 25 nucleotides long
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