Affymetrix genechip array station
This presentation is the property of its rightful owner.
Sponsored Links
1 / 20

AFFYMETRIX GENECHIP ARRAY STATION PowerPoint PPT Presentation


  • 92 Views
  • Uploaded on
  • Presentation posted in: General

AFFYMETRIX GENECHIP ARRAY STATION. By. Pierce Chuang, Vishu Rajamani, Jeremy Rasiah. Overview. Introduction to DNA/RNA Sequencing Techniques Affymetrix GeneChip Station GeneChip Station Architecture Manufacturing Process Questions. Introduction to DNA/RNA. Introduction DNA/RNA (cont’d).

Download Presentation

AFFYMETRIX GENECHIP ARRAY STATION

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript


Affymetrix genechip array station

AFFYMETRIX GENECHIP ARRAY STATION

By

Pierce Chuang, Vishu Rajamani, Jeremy Rasiah


Overview

Overview

  • Introduction to DNA/RNA

  • Sequencing Techniques

  • Affymetrix GeneChip Station

  • GeneChip Station Architecture

  • Manufacturing Process

  • Questions


Introduction to dna rna

Introduction to DNA/RNA


Introduction dna rna cont d

Introduction DNA/RNA (cont’d)


Genes

Genes


Dna sequencing techniques

DNA Sequencing Techniques

  • Chain-terminator method (Sanger)

    • Dideoxynuclotides triphosphates (ddNTPs) as DNA chain terminators

    • Sequence roughly 550,000 bases in a day

    • Requires thirty years of uninterrupted operation to sequence all nucleotides in the human genome


Dna sequencing techniques1

DNA Sequencing Techniques

  • Sequencing By Hybridization (SBH)

    • Ex, target DNA sequence, AGCCTAGCTGAA

Affymetrix Microarray Specification


Affymetrix genechip station

Affymetrix GeneChip Station


Assay preparation

Assay Preparation


Probes

Probes


Genechip array

GeneChip Array

source: http://bach2.biosci.arizona.edu/


Results

Results

source: http://www.med.upenn.edu/


Affymetrix genechip station architecture

Affymetrix GeneChip Station Architecture


Detector architecture

Detector Architecture

source: http://www.affymetrix.com/


Genechip array1

GeneChip Array

source: http://bach2.biosci.arizona.edu/


Genechip array cross section

GeneChip Array Cross Section


Array manufacturing process

Array Manufacturing Process

  • 2: substrates, SiO2, Si, GaAs, or Glass.

  • 6: Linker Molecules. Ex, Aryl Acetylene

  • 8: Mask, for photolithographic process

  • 10: Activated Linker Molecules


Array manufacturing process1

Array Manufacturing Process


Array manufacturing process2

Array Manufacturing Process

  • This process is repeated several times to produce longer probes.

  • Full length probes are usually 25 nucleotides long


Questions

Questions ?


  • Login