The Mutability and Repair of DNA
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The Mutability and Repair of DNA. REPLICATION ERRRORS AND THEIR REPAIR. The nature of mutations. Transitions. Transversions. Some replication errors escape proofreading. Mismatch repair removes errors that escape proofreading. Structure of MutS and DNA

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The Mutability and Repair of DNA


The nature of mutations



Structure of MutS and DNA

(MutS recognizing mismatches from the distortion they cause)

Dam methylation in E. coli replication fork


DNA undergoes damage spontaneously from hydrolysis and deamination

Thymine dimer caused by UV light

Ames test for in DNAcarcinogens

Bruce N. Ames in DNA

Professor of the Graduate School Division of Biochemistry and Molecular Biology, UC Berkeley

Structure of DNA- base-flipping mechanismglycosylase complex

How is an altered base detected within the context of the double-helix?

The recognition of an unusual nucleotide in DNA by base-flipping of glycosylase

Oxo double-helix?-G:A repair by a fail-safe glycosylase

Transcription-coupled DNA repair either side of the lesion

Recombination repair DNA breaks by retrieving sequence information from undamaged DNA

Double-Strand Breaks are Efficiently Repaired

Translesion ends DNA synthesis enables replication to proceed across DNA damage

Structures of a endstranslesion polymerase and a typical polymerase