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N -Glycosylation mediation of Plant Immunity in Arabidopsis Matt Borchardt and Jessica Allen

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N -Glycosylation mediation of Plant Immunity in Arabidopsis Matt Borchardt and Jessica Allen. Max-Planck Society for the Advancement of Science. Originally created as the Kaiser Wilhelm Society in 1911, changed in 1948 Boasts 32 Nobel Prizes awarded to their scientists

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max planck society for the advancement of science
Max-Planck Society for the Advancement of Science
  • Originally created as the Kaiser Wilhelm Society in 1911, changed in 1948
  • Boasts 32 Nobel Prizes awarded to their scientists
  • Top Non-University Institution in Science by Times Higher Education
  • Albert Einstein, Fritz Haber, and Peter Debye were named directors at some point in its history

http://en.wikipedia.org/wiki/Max-Planck-Gesellschaft

slide4

Arabidopsis

    • Brassicaceae family
    • model organism
      • ABC Plant Model
    • First plant to have entire genome sequenced

http://en.wikipedia.org/wiki/Arabidopsis

signaling in cells
Signaling in Cells
  • Cells are constantly being introduced to various signals inside and outside of the cell
    • Signal receptors are signal-specific
    • Causes a cellular response
example of cell signaling
Example of Cell Signaling

219.221.200.61/ywwy/zbsw(E)/edetail6.htm

cell signaling and plants
Cell Signaling and Plants
  • Cell signaling is important for Plant Immunity
  • Provides feedback systems to promote cellular defense
    • Growth inhibition
    • Production of Reactive Oxygen Species (ROS)
      • “oxidative burst”
    • Apoptosis
plant receptors
Plant Receptors
  • Pattern Recognition Receptors (PRRs)
    • membrane-bound protein of the innate immune system
    • recognize microbe-associated molecular patterns (MAMPs) on pathogens
    • Contain membrane-bound polysaccharides
slide9

Arabidopsis has approximately 56 known RLPs

    • EFR recognizes and responds to elf18
    • FLS2 recognizes and responds to flg22
  • MAMPs are recognized by N-glycans on PRRs
slide10

N-Glycans

    • Attach to asparagine residues of plasma membrane
    • Initial contact between plant cells and pathogens
n glycosylation
N-glycosylation
  • Occurs in eukaryotes during/after translation
  • Linking of 14-sugar precursor to the asparagine in the polypeptide chain

12-52.Molecular Biology of the Cell, 4th Edition

oligosaccharyltransferase complex
Oligosaccharyltransferase Complex
  • Transfers the 14-sugar oligosaccharide from dolichol to the protein
  • The central step in N-linked glycosylation
  • Located in the ER
  • Post-translational modification

http://www.ier.tokushima-u.ac.jp/bunshi-seiri/physiol/images/PfSTT3.jpg

altered prevention of n glycosylation
Altered/Prevention of N-Glycosylation
  • Creates unfolding of proteins
  • Only correctly folded proteins are exported from the ER
  • Causes a reduction in receptor accumulation
    • Causes lack of receptor-signal induced pathways
    • Leads to decreased immune response
in this study
In this study
  • Mutated genes produce plants with modifications in the N-glycosylation steps
    • stt3a
  • Genes present in ER which encode for oligosaccharyltransferase complex
tunicamycin
Tunicamycin
  • Antibiotic
  • Blocks glycoprotein synthesis in first step of glycosylation
  • Causes arrest of cell cycle in G1
  • Produced by bacterium Streptomyces

http://fermentek.co.il/tunicamycin.htm

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MAMP

MAMP

Bacteria

Bacteria

PRR-EFR

PRR-FLS2

Plasma Membrane

Seedling Growth

ROS

Endoplasmic Reticulum

Golgi Apparatus

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