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N -Glycosylation mediation of Plant Immunity in Arabidopsis Matt Borchardt and Jessica Allen. Max-Planck Society for the Advancement of Science. Originally created as the Kaiser Wilhelm Society in 1911, changed in 1948 Boasts 32 Nobel Prizes awarded to their scientists

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N-Glycosylation mediation of Plant Immunity in Arabidopsis

Matt Borchardt and Jessica Allen


Max planck society for the advancement of science
Max-Planck Society for the Advancement of Science

  • Originally created as the Kaiser Wilhelm Society in 1911, changed in 1948

  • Boasts 32 Nobel Prizes awarded to their scientists

  • Top Non-University Institution in Science by Times Higher Education

  • Albert Einstein, Fritz Haber, and Peter Debye were named directors at some point in its history

http://en.wikipedia.org/wiki/Max-Planck-Gesellschaft


  • Arabidopsis

    • Brassicaceae family

    • model organism

      • ABC Plant Model

    • First plant to have entire genome sequenced

http://en.wikipedia.org/wiki/Arabidopsis


Signaling in cells
Signaling in Cells

  • Cells are constantly being introduced to various signals inside and outside of the cell

    • Signal receptors are signal-specific

    • Causes a cellular response


Example of cell signaling
Example of Cell Signaling

219.221.200.61/ywwy/zbsw(E)/edetail6.htm


Cell signaling and plants
Cell Signaling and Plants

  • Cell signaling is important for Plant Immunity

  • Provides feedback systems to promote cellular defense

    • Growth inhibition

    • Production of Reactive Oxygen Species (ROS)

      • “oxidative burst”

    • Apoptosis


Plant receptors
Plant Receptors

  • Pattern Recognition Receptors (PRRs)

    • membrane-bound protein of the innate immune system

    • recognize microbe-associated molecular patterns (MAMPs) on pathogens

    • Contain membrane-bound polysaccharides



  • N-Glycans

    • Attach to asparagine residues of plasma membrane

    • Initial contact between plant cells and pathogens


N glycosylation
N-glycosylation

  • Occurs in eukaryotes during/after translation

  • Linking of 14-sugar precursor to the asparagine in the polypeptide chain

12-52.Molecular Biology of the Cell, 4th Edition


Oligosaccharyltransferase complex
Oligosaccharyltransferase Complex

  • Transfers the 14-sugar oligosaccharide from dolichol to the protein

  • The central step in N-linked glycosylation

  • Located in the ER

  • Post-translational modification

http://www.ier.tokushima-u.ac.jp/bunshi-seiri/physiol/images/PfSTT3.jpg



Altered prevention of n glycosylation
Altered/Prevention of N-Glycosylation

  • Creates unfolding of proteins

  • Only correctly folded proteins are exported from the ER

  • Causes a reduction in receptor accumulation

    • Causes lack of receptor-signal induced pathways

    • Leads to decreased immune response


In this study
In this study

  • Mutated genes produce plants with modifications in the N-glycosylation steps

    • stt3a

  • Genes present in ER which encode for oligosaccharyltransferase complex


Tunicamycin
Tunicamycin

  • Antibiotic

  • Blocks glycoprotein synthesis in first step of glycosylation

  • Causes arrest of cell cycle in G1

  • Produced by bacterium Streptomyces

http://fermentek.co.il/tunicamycin.htm


MAMP

MAMP

Bacteria

Bacteria

PRR-EFR

PRR-FLS2

Plasma Membrane

Seedling Growth

ROS

Endoplasmic Reticulum

Golgi Apparatus


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