Workpackage 02 Germination testing Christoph Germeier ,

1. Workpackage 02 Germination testing Christoph Germeier, Zofia Bulinska – Radomska, Izabela Borowska, Audrey Didier

2. Germination methodology • Germination and Fusarium counting methodology • Germination test results 2008 • Germination methodology check test • Germination test results 2009 • germination test: how many accessions are being tested, when the tests have been started, how long do they all take?

3. Germination methodology General outline of germination tests according to ISTA methodology: Between paper in climate chamber, Continuous light 20°C, 4 replications (recommended 8) a 50 seeds Breaking dormancy with gibberellic acid: GA3 (1000 mg/l) in phosphate buffer (1.7799 g Na2HPO4 x 2 H20 + 1.3799 g NaH2PO4 x H2O + 1 l aqua dest.) Count after 5 and 10 days Variants used in the project FRA 2007 / 2008: Round filter paper (diameter: 90mm) are moistened with gibberellin solution and placed in the petri plate then seeds are laid down onto the filter paper. A second moistened round filter paper is disposed on the top. POL 2007 / 2008: Filter paper envelopes (CHEMPUR, 65g/m2, 12,5x18,5 cm) are moistened with gibberellin solution. Seeds are placed into the envelope, incubated at 200C with continuous light.

4. Germination methodology: Radzików

5. Germination methodology: Radzików Mold Normal germ

6. Germination methodology: Radzików Fusarium Normal germ

7. DEU 2007 / 2008: Filter paper towels (e.g. Munktell C160, 15x60cm2) are pre-folded and moistened with gibberellin solution. Seeds are placed onto the fold, the towel then folded, rolled, stabilized in a plastic bag and incubated at 200C with continuous light. 2007: climate cabinet side of plastic bag open 2008: climate chamber top of plastic bag open

8. Germination methodology: Quedlinburg Set up of germination tests in climate chamber (2008)

9. Germination and Fusarium counting methodology: Quedlinburg • A) Abnormal germ and Molds

10. Germination and Fusarium counting methodology: Quedlinburg • B) Fusarium more or less thick mycelium, reddish color, plants affected or not germinated

11. Germination and Fusarium counting methodology: Quedlinburg • B) Fusarium more or less mycelium, reddish colour, plants not affected

12. Germination and Fusarium counting methodology: Quedlinburg • B) Fusarium mainly reddish color, mycelium, symptoms not detectable

13. Germination methodology check test (wild species)

14. Germination methodology check test (marginally cultivated species)

15. Germination methodology check test (cultivated oat) • Germination methodology check test

16. Germination tests total summary

17. Germination results species wise – wild species

18. Germination results species wise

19. Fusarium [%] results species wise – wild species

20. Fusarium results [%] species wise – cultivated species

21. Germination check results compared with germination test 2007/2008

22. Germination check results compared with germination test 2007/2008

23. Conclusion Main problem reducing germinability: Excess of water!

24. Thanks for your attention