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Lysteria monocytogenes and Shigella flexerni use actin based motility to spread to other cells PowerPoint PPT Presentation


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Lysteria monocytogenes and Shigella flexerni use actin based motility to spread to other cells. -Cause diarrhea, vomiting, fever, and can lead to menningitis and spontaneous abortions -Causes death in immunocompromised people (children, elderly, AIDS patients)

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Lysteria monocytogenes and Shigella flexerni use actin based motility to spread to other cells

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Lysteria monocytogenes and shigella flexerni use actin based motility to spread to other cells

Lysteriamonocytogenesand Shigellaflexerni

use actin based motility to spread to other cells

-Cause diarrhea, vomiting, fever, and can lead to menningitis and spontaneous abortions

-Causes death in immunocompromised people (children, elderly, AIDS patients)

-Produce a protein that encourages production of actin filaments at the cell surface

-Propels bacteria through the cytoplasm (Comet tails)

-Rams into the cytoplasmic membrane and pushes through into the next cell

-Avoids much of the immune system

http://cmgm.stanford.edu/theriot/movies.htm#Hits

Movies:


Lysteria monocytogenes and shigella flexerni use actin based motility to spread to other cells

Vibriofischeri and its host, the bobtail squid

Close up of single bacterium (Vibrio)

Bacteria (Vibrio) glowing on a petri dish

Light organs on squid belly use

Vibrio to reflect the same

wavelength of light as the moon, hiding their shadow from preditors

A squid that uses glowing bacteria

to avoid being eaten (the glow from

the bacteria mimics the moonlight so the

squid can’t be seen from below-just looks

like moonlight from below)


Lysteria monocytogenes and shigella flexerni use actin based motility to spread to other cells

Vibrioand its host, the Pineapple fish

Pineapple fish-lower jaw contains a photophore where

Vibrio reside. The bioluminescence attracts zooplankton.

-glows orange during the day, blue green by night.


Lysteria monocytogenes and shigella flexerni use actin based motility to spread to other cells

The angler fish uses bioluminescent bacteria to attract prey


Lysteria monocytogenes and shigella flexerni use actin based motility to spread to other cells

Quorum sensing in Vibrio

http://subrealism.blogspot.com/2009_04_01_archive.html

Bioluminescence is very expensive so the cells only want to bioluminesce when

other cells are around. No point if they’re not in the squid light organ.


Lysteria monocytogenes and shigella flexerni use actin based motility to spread to other cells

The amazing Myxococcusxanthus


Lysteria monocytogenes and shigella flexerni use actin based motility to spread to other cells

Myxococcus

Myxococcusxanthus cell

A Myxococcusswarm


Lysteria monocytogenes and shigella flexerni use actin based motility to spread to other cells

Aggregation and “rippling” of Myxococcus


Lysteria monocytogenes and shigella flexerni use actin based motility to spread to other cells

Fruiting bodies

Myxococcusfruiting bodies

Dictyostellumdiscoideum-slime mold fruiting bodies (amoeba)


Lysteria monocytogenes and shigella flexerni use actin based motility to spread to other cells

Slime mold fruiting bodies

Dictyostellumdiscoideum-slime mold fruiting bodies (amoeba)


Lysteria monocytogenes and shigella flexerni use actin based motility to spread to other cells

Sporulation sites on the surfaces of WT colonies.

Bacillus fruiting bodies

Bacillus fruiting body. A sporulation gene was fused with lacZ

and the cells were plated on medium containing x-gal. You can

see the where sporulation occurs by the dark blue color

Branda S S et al. PNAS 2001;98:11621-11626


Lysteria monocytogenes and shigella flexerni use actin based motility to spread to other cells

Movies

http://www.youtube.com/watch?v=oVGEFGzS7Wo&feature=related

http://www.youtube.com/watch?v=O1jPzhz1Qyc

http://www.youtube.com/watch?v=bkVhLJLG7ug&feature=related


Lysteria monocytogenes and shigella flexerni use actin based motility to spread to other cells

Healthy ant


Lysteria monocytogenes and shigella flexerni use actin based motility to spread to other cells

Zombie ants


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