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Chapter 16

Chapter 16. Chapter 16. In General…… What is the definition of volume and what is the basic unit? How do you use a graduate cylinder to measure volume? What are the instruments used to measure volume? In order of increasing accuracy, list the instruments that are used to measure volume.

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Chapter 16

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  1. Chapter 16

  2. Chapter 16 • In General…… • What is the definition of volume and what is the basic unit? • How do you use a graduate cylinder to measure volume? • What are the instruments used to measure volume? • In order of increasing accuracy, list the instruments that are used to measure volume. • How are micropipettors validated?

  3. Glassware • Calibrated either TC or TD-what does this mean? • What are the tolerances for a volumetric in comparison to a graduated cylinder? • Compare and contrast the use of volumetrics, graduated cylinders, beakers and flasks • What is a serological pipet? • What is a Mohr pipet? • What is a Pasteur pipet?

  4. Micropipetting Devices • Positive displacement vs air displacement

  5. Pipetting of Difficult Solutions • Air displacement micropipettors are not very accurate when dispensing volatile liquids (like acetone) or viscous materials (like protein solutions). • Review Table 16.3

  6. Validation of Micropipettors • Box 5 pages 314-315 • Problems • All

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