Ferrophilic Characteristics of Vibrio vulnificus

1. Ferrophilic Characteristics of Vibrio vulnificus and Potential Usefulness of Iron Chelation Therapy Choon-Mee Kim, Ra-Young Park, Mi-Hwa Choi, Hui-Yu Sun, and Sung-Heui Shin The Journal of Infectious Diseases 2007; 195:90–8 2007.11.28 Speaker : 郭 懿 瑩

3. Requirement of non–transferrin-bound iron for Vibrio vulnificus growth initiation in a human ex vivo background FIG.1 The growth of MO6-24/O was more profoundly stimulated by FC than by HT

4. Requirement of non–transferrin-bound iron for the initiation of Vibrio vulnificus growth and efficient transferrin-bound iron use FIG.2 These results indicate that V.vulnificus cannot use TBI as an iron source for growth initiation under severely NTBI-limited conditions—that is, NTBI is essentially required for efficient TBI use by and the growth initiation of V. vulnificus.

5. Expression of the vulnibactin-mediated iron-uptake system and growth initiation of V. vulnificus FIG.3 Expression of the vulnibactin-mediated iron-uptake system is not essentially required for the initiation of V. vulnificus growth, especially at low initial bacterial densities.

6. Relative iron requirements of V. vulnificus FIG.4 V. vulnificus requires a higher level of readily available iron for the initiation of growth than E. coli or S. aureus.

7. Relative ability of Vibrio vulnificus to produce siderophores and to use transferrin-bound iron. FIG.5 V. vulnificus cannot assimilate iron from transferrins as effectively as E. coli or S. aureus because of its relative inability to produce siderophores.

8. Effect of deferiprone and deferoxamine on the growth of Vibrio vulnificus FIG.6 These results indicate that deferiprone, but not deferoxamine, inhibits the growth of V. vulnificus by reducing the level of readily available iron at low concentrations.

9. These results show that V. vulnificus is a ferrophilic bacterium that requires higher NTBI levels than other pathogens and that iron chelation therapy might be an effective means of preventing the in vivo growth of V. vulnificus in susceptible patients.