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Vector pS1301 carrying chimeric gene PowerPoint PPT Presentation


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BamH I. EcoR I. LB. Hpt. P 35S. TEVL. Chimeric gene. NOS. GUS. RB. Vector pS1301 carrying chimeric gene.

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Vector pS1301 carrying chimeric gene

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Vector ps1301 carrying chimeric gene

BamHI

EcoRI

LB

Hpt

P35S

TEVL

Chimeric gene

NOS

GUS

RB

Vector pS1301 carrying chimeric gene

SupplementaryFigure 1. Schematic diagrams of the transformation construct. RB and LB, right and left T-DNA border; GUS, -glucuronidase gene; Hpt, hygromycin phosphotransferase gene; P35S, cauliflower mosaic virus 35S promoter; TEVL, 5‘-nontranslated region of the tobacco etch virus; NOS, nopaline synthase polyadenylation signal. Four chimeric genes, D52S, D56S, D57S, and D92S, were cloned into this vector.


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