1 / 4

Identification of an AFLP marker for stem rust resistance in Avena strigosa

Identification of an AFLP marker for stem rust resistance in Avena strigosa. Taye Zegeye Daryl Somers Thomas Fetch Jr. Lakhdar Lamari. AAFC-CRC, Winnipeg. June 30, 2008. Materials and Methods. Two F 2 populations produced DNA extracted - Qiagen DNeasy 96 plant kit

nuncio
Download Presentation

Identification of an AFLP marker for stem rust resistance in Avena strigosa

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Identification of an AFLP marker for stem rust resistance in Avena strigosa Taye Zegeye Daryl Somers Thomas Fetch Jr. Lakhdar Lamari AAFC-CRC, Winnipeg June 30, 2008

  2. Materials and Methods • Two F2 populations produced • DNA extracted - Qiagen DNeasy 96 plant kit • AFLP fingerprinting technique • Genomic DNA digested - PstI andMseI • BSA performed - 256PstI+MseIprimer pairs • PCR • ABI 3100 - Genetic Analyzer • A genetic map produced - JoinMap

  3. Results F2 population from CN56979/CN57130 Primers: P-acg+M-cga RP SP M 4 82 56 90 400 370 350 340 300 250 200 160 150 135

  4. Results - Mapping 0 PaatMccg15 2 PaaaMcga285 5 PaaaMcga 8 PaggMcaa155 15 PaggMcaa205 16 PaggMcaa190 22 PaccMcgg230 28 PacgMcga370 37 Sr_57130 62 PaagMctc140 63 PaagMctc130 64 PaaaMcga330 66 PaagMctc110 71 PacgMcga120

More Related