Progress report 13.06.05. RESULTS and FUTURE ACTIVITIES. FOTON experiments: growth curves. P. putida in FOTON medium (defined M9Glu medium supplemented with 20% LB medium) at 30°C (shaking); inoculum 10 6 cfu/ml. Results similar for other bacteria.
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Progress report 13.06.05
RESULTS and FUTURE ACTIVITIES
P. putida in FOTON medium (defined M9Glu medium supplemented with 20% LB medium) at 30°C (shaking); inoculum 106 cfu/ml.
Results similar for other bacteria
The experiment from the FOTON flight will be replicated on Earth following the indications of the temperature data logger
Adhesion on material will be determined either by staining and microscopic analysis or by quantitative FISH; space and Earth samples will be compared
Fluorescence staining of a steel coupon colonized by P. putida
Experiments performed in the first part of the project point to synergistic biofilm formation by mixed bacterial populations
1 2 3 4 5 6
1 2 3 4 5 6
Sedimentation due to cell aggregation
Reduced sedimentation in non-aggregative strains
(Experiment by E. Brombacher; protocol as in Simm et al., 2004)