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Disque de Secchi

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Disque de Secchi. Rosette + CTD. Filet a plancton. Bouteille NISKIN. Slurp gun. Algues des glaces. Image satellitaires SeaWifs. Fixation des échantillons de phytoplancton : -Lugol -Formol -Glutaraldehyde. Enumeration du phytoplancton :.

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Presentation Transcript
slide1

Disque de Secchi

Rosette + CTD

Filet a plancton

Bouteille NISKIN

slide2

Slurp gun

Algues des glaces

slide4

Fixation des échantillons de phytoplancton:

-Lugol

-Formol

-Glutaraldehyde

Enumeration du phytoplancton:

Préparation des échantillons d’eau pour la mesure de la teneur en pigment:

-filtration sur support GF/C ou GF/F

-extraction dans solvant (90% acétone, méthanol, gradient) au noir plusieurs heures au froid (4C ou -20C)

-mesure de la fluorescence ou de l’absorption des pigments chlorophylliens a une ou plusieurs longueurs d’onde

slide5

Fluoromètre

Spectrophotomètre

HPLC

slide7

picoeukaryotes

Synechococcus

Fluorescent beads (1 µm)

Prochlorococcus

picoeukaryotes

  • nanoeukaryotes
  • 6-10 microns
  • 3-6 microns

Red fluo. (au)

Red fluo. (ua)

Synechococcus

Green fluo.(au)

RALS(au)

Cytometrie en flux

  • Phycoerythrine vs FS
  • ex : 488 nm
  • Orange fluo.

Cyanobacteria, Cryptophytes

  • Phycocyanine vs FS
  • ex : 633 nm
  • Red fluo.

Cyanobacteria, Cryptophytes

  • Clorophyll vs FS
  • ex : 488 nm
  • Red fluo.

All Pytoplankton

Cryptophytes

slide8

Filet a plancton

Pompe

Bongo

Systeme fermant

WP2

slide9

Filet a nappes

Niskin

Epineuston

Blue water diving

Sousmarin

et

Appareil teleguide

slide13

Vertical profilers

Underwater Video Profiler

Zoovis

slide14

LAPIS

(Large Area Plankton Imaging System)

slide16

Flowcam

Zooscan