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Experiment A: Ergebnisse der X-gal-Topagar-Detektion der -Galaktosidase-Aktivität aller in der ersten Serie isolierten Mutanten. SD-TRP-URA. SD-TRP-URA. X-gal –LEU-TRP. Gr. 1. Gr. 2. Gr. 3. Gr. 4. Gr. 5. Gr. 6. Gr. 7. wt. 1 2 3 4 5 6. Experiment A:

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  1. Experiment A: Ergebnisse der X-gal-Topagar-Detektion der -Galaktosidase-Aktivität aller in der ersten Serie isolierten Mutanten SD-TRP-URA SD-TRP-URA X-gal –LEU-TRP Gr. 1 Gr. 2 Gr. 3 Gr. 4 Gr. 5 Gr. 6 Gr. 7 wt 1 2 3 4 5 6

  2. Experiment A: Ergebnisse der X-gal-Topagar-Detektion der -Galaktosidase-Aktivität aller in der zweiten Serie isolierten Mutanten 1 2 3 4 5 6 7 8 9 10 11 12 Gr. 1 Gr. 2 Gr. 3 Gr. 4 X-gal –LEU-TRP Gr. 5 Gr. 6 Gr. 7 13-1 13-2 13-3 13-4 13-5 13-6 13-7 wt wt wt wt wt Die Auswertung der Kreuzung dieser Serie dauert noch an.

  3. Experiment C: Cycloheximide Chase Analyse von DHFR-mutD-HA CDC11 mutD-ha2 CDC11 mutD-ha2 CDC11 mutD-ha2

  4. Experiment C: Quantifizierung der Western Blot Signale von DHFR-mutD-HA normalisiert gegen die CDC11-Signale CDC11 DHFRmutD-HA

  5. PGK Pup1 Experiment C: Quantifizierung der Western Blot Signale von PUP1/2 0 1 2 ON ON = over night

  6. mutant DHFR proteins fused to GFP form microscopically detectable aggregates DHFRwt-GFP DHFRmutC-GFP no aggregates cells often containd single aggregates

  7. GBD-ODC GBD GAD-Oaz1 GAD-ODC GAD SD ohne Leu, Trp, His SD ohne Leu, Trp, Ade Two hybrid assay Versuch E SD ohne Leu, Trp Kreuzungen nach Replika

  8. 1 2 3 4 5 6 7 8 9 10 anti-alpha7 1 JD47-13C ("Wildtyp") 2 ump1∆ 3 beta1 4 beta2 5 beta3 6 beta4 7 beta5 8 beta6 9 beta7 10 UMP1-HA Versuch C 1 2 3 4 5 6 7 8 9 10 anti-beta2 1 2 3 4 5 6 7 8 9 10 anti-Rpt5

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