Chili Peppers: A Cure for Breast Cancer? Treating MCF-7 Breast Cancer Cells with Capsaicin at Varying Concentrations Andrew Sutton Department of Biology, York College of Pennsylvania. http://en.wikipedia.org/wiki/File:Kapsaicyna.svg. Introduction. Methods.
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Treating MCF-7 Breast Cancer Cells with Capsaicin at Varying Concentrations
Department of Biology, York College of Pennsylvania
Figure 4. Treatment of MCF-7 Cells with Capsaicin at various mM concentrations
H0:Treating MCF-7 breast cancer cells with Capsaicin
will not cause cell death.
H1: Treating MCF-7 breast cancer cells with Capsaicin will induce cell death through apoptosis.
Figure 5. Caspase3 Assay of Varying Levels of Capsaicin Treated MCF-7 cells
after 24 hours. 500 μM Capsaicin treatment showed a significant difference according
to an unpaired t-test. P-value <0.05
Figure 1. MCF-7 cells in culture dishes prior to various Capsaicin treatments for Caspase3 Assay.
Ito, Keisuke., Nakazato, Tomonori., Yamato, Kenji, 2004. Induction of Apoptosis in Leukemic Cells by HomovanillicAcid Derivative, Capsaicin, through Oxidative Stress: Implication of Phosphorylation of p53 at Ser-15 Residue by Reactive Oxygen Species. Cancer Research [serial online] 64: 1071-1078.
Mori, Akio., Lehmann, Soren., O’Kelly, James, 2006. Capsaicin, a Component of Red Peppers, Inhibits the Growth of Androgen-Independent, p53 Mutant Prostate. Cancer Research [serial online] 66: 3222-3229.
Figure 2. MCF-7 cells growing and dividing at 400X magnification (left) and 200X (right).
Figure 3: MCF-7 cells after treatment with Capsaicin at varying concentrations.
Thank you Dr. Thompson for all your guidance on this project. Thank you to Joan Carpenter for obtaining the MCF-7 cells