Announcements, Feb. 9. Reading for today: 154-171 on membrane lipids. Reading for Monday: 172-186 on membrane proteins. Reading for Wednesday: 191-207 on membrane transport. Reading for Friday: 207-216 on energetics of membrane transport. I. Membrane lipids Membrane functions
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Isolating membrane lipids
Historical models of membranes
Fluid mosaic model
Evidence concerning lipid part of membrane
After reading the text, attending lecture, and reviewing lecture notes, you should be able to
List various functions of membranes.
Explain how thin-layer chromatography (TLC) can be used to fractionate lipids.
Compare historical models of membrane structure.
Describe experimental evidence for membrane lipid composition, structure and fluidity.Outline/Learning Objectives
Lyse w/ dH2O, centrifuge
Plasma membrane “ghosts”
Extract w/ chloroform-MeOH
Lipids separated by polarity:
least travels farthest
Proteins separated by size:
smallest travel farthestMembranes: How would you study them?
bone is serine
Interpretation membranes2. Evidence for Lipid Bilayer:X-ray crystallography of Membranes
More fluid →
Less fluid →
Cells can regulate
Atomic force microscopy reveals sphingomyelin rafts (orange) protruding from a PC background (black) in a mica-supported lipid bilayer. Placental alkaline phosphatase (yellow peaks), a GPI-anchored protein, is shown to be almost exclusively raft-associated. For details see the article by Saslowsky et al. J. Biol. Chem. 277, Cover of #30, 2002.
A antigen Membrane
HH or Hh
Precursor H substance
B antigenCHO modification of Glycolipids:The ABO blood groups
ABO Blood Groups Membrane
A - A antigen only
B - B antigen only
AB - Both A and B antigens
O - Neither antigen