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ccdB

ccdB. ccdB. ccdB. ccdB. pOp6. pOp6. RB. RB. LB. LB. nos. 35S. int. int. GUS. GUS. hyg. kan. T. T. T. T. ccdB. ccdB. pOp6. RB. LB. 35S. 35S. LhGR. int. GUS. hyg. T. T. T. ccdB. ccdB. pOp6. RB. LB. 35S. nos. LhGR. int. GUS. nptII. T. T. T. 0.8. 1.4.

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ccdB

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  1. ccdB ccdB ccdB ccdB pOp6 pOp6 RB RB LB LB nos 35S int int GUS GUS hyg kan T T T T ccdB ccdB pOp6 RB LB 35S 35S LhGR int GUS hyg T T T ccdB ccdB pOp6 RB LB 35S nos LhGR int GUS nptII T T T 0.8 1.4 ~6.2 Xba1 Xba1 Xba1 Xba1 pOpOff1(hyg) XhoI KpnI KpnI 3.5 1.4 1.4 Xba1 Xba1 pOpOff1(kan) XhoI KpnI 1.4 0.8 1.4 ~6.0 1.5 1.8 Xba1 Xba1 Xba1 Xba1 Xba1 Xba1 pOpOff2(hyg) XhoI XhoI XhoI KpnI KpnI 3.5 1.4 ~4.6 1.2 1.4 ~6.0 1.5 Xba1 Xba1 Xba1 Xba1 pOpOff2(kan) XhoI XhoI XhoI KpnI 1.4 ~4.6 1.2 Maps of pOpOff vectors with suggested sites for diagnostic restriction digests following recombination of target gene fragments to replace the ccdB gene. The vector backbones (not shown) are >10kb in size. Restriction fragment sizes are approximate and given in kb.

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