A piggyBac transposon A short inverted-repeat-type DNA transposable element : This DNA sequence has a propensity for

1. A piggyBac transposon (A short inverted-repeat-type DNA transposable element) : This DNA sequence has a propensity for jumping into other genes => The piggyBac transposon is used in genetic engineering of insects

2. The piggyBac transposon is flanked by short inverted repeats the ORF of piggyBac transpon encodes a transposase piggyBac relies on a transposase enzyme to help it move The transposon can cause a mutation or act as a recombination site leading to duplication and deletion of sequences in the chromosomes

3. Figure: 16.2 Title: Transposons have inverted repeats and generate target repeats Caption: Transposons have inverted terminal repeats and generate direct repeats of flanking DNA at the target site. In this example, the target is a 5 bp sequence. The ends of the transposon consist of inverted repeats of 9 bp, where the numbers 1 through 9 indicate a sequence of base pairs. Figure: 16.2 Title: Transposons have inverted repeats and generate target repeats Caption: Transposons have inverted terminal repeats and generate direct repeats of flanking DNA at the target site. In this example, the target is a 5 bp sequence. The ends of the transposon consist of inverted repeats of 9 bp, where the numbers 1 through 9 indicate a sequence of base pairs.

4. The uses of piggyBac - Introducing genes to create sterile insects for use in sterile-release pest control programs.

5. to specifically pick mosquito transposons or something that would be expected to be very similarto Aedes aegypti transposons. Doing the blasting on the nucleotides could be valuable. � Using a more powerful method than hmmer and blast -- neural nets, contextfree grammars, graph algorithms, etc.

6. Figure: 16.1 Title: DNA rearrangements have many causes Caption: A major cause of sequence change within a genome is the movement of a transposon to a new site. This may have direct consequences on gene expression. Unequal crossing-over between related sequences causes rearrangements. Copies of transposons can provide targets for such events. Figure: 16.1 Title: DNA rearrangements have many causes Caption: A major cause of sequence change within a genome is the movement of a transposon to a new site. This may have direct consequences on gene expression. Unequal crossing-over between related sequences causes rearrangements. Copies of transposons can provide targets for such events.