Boys and girls
This presentation is the property of its rightful owner.
Sponsored Links
1 / 45

Boys and girls Welcome PowerPoint PPT Presentation


  • 94 Views
  • Uploaded on
  • Presentation posted in: General

Boys and girls Welcome. About practical lessons. 1 Lab: morphology lab 5 and 6 On fourth floor of east building class one in lab5, class two in lab6 2 Preparation: Textbooks laboratory manual practical paper and so on 3 no food in lab. Exercises after class.

Download Presentation

Boys and girls Welcome

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript


Boys and girls welcome

Boys and girls

Welcome


About practical lessons

About practical lessons

1 Lab: morphology lab 5 and 6

On fourth floor of east building

class one in lab5,

class two in lab6

2 Preparation:

Textbooks

laboratory manual

practical paper and so on

3 no food in lab


Exercises after class

Exercises after class

1 HE staining

Acidophilc,

Basophilc

2 Features of electron microscopy

Tissue slide in practical lessons:

Spinal ganglion


Boys and girls welcome

Self Introduction

Zhao Hongxian

Lecturer

Master graduate

Email:[email protected]

QQ:15673656


Boys and girls welcome

Faculty of

the department of histology and embryology


Chapter 1 histological introduction

第一章 组织学绪论

Chapter 1 Histological Introduction


Boys and girls welcome

2 Significants(why)

3 Technology(how)

Main contents:

1 Definiton、contents

(what)


Boys and girls welcome

1 Definiton,study contents

1.1 Definiton of histology(组织学)

Histo = tissue, logos = study or science

A science which studies

the normalmicrostructures of the body

and the basic functions.


1 2 study contents

1.2 Study contents

Cell(细胞):

Basic unit of structure and function of body

Tissue(组织):

★Definition: made of cells

and extracellular matrix

★Types:(1) Epithelial tissue,Epi

(2) Connective tissue, CT

(3) Muscle tissue

(4) Nervous tissue

3. Organ(器官)and system(系统)


Boys and girls welcome

Differences?


Boys and girls welcome

2 Significants

Basic science:WHY?

Precondition of studying other medical disciplines, especially physiology

and pathology


Boys and girls welcome

Intern (exercitation)

Graduate

Clinic (Medicine, Surgery, Gynecology, Pedology, etc.)

Pathphysiology

Pharmocology

Basic medicine &Surgery

Biochemistry

Physiology

Microbiology

Immunology

Pathology

Parasitology

macrostructure

microscope

First

Year

Histology & Embryology

Anatomy


Boys and girls welcome

3 Histological techniques

3.1 Observing tools(your weapon)

光镜(light microscope, LM)

电镜(electron microscope, EM)

3.2 Samples

paraffin section, frozen section,

ultrathin section ,Smear, stretched preparation,

ground section, living cell.

3.3 Treatments of sample

HE staining, special staining,, histochemistry, in situ hybridization, heavy metal staining, autoradiography vital staining


Boys and girls welcome

3.1 observing tools

3.1.1 Light microscope

Based on the interaction of

light and tissue components

Compositions:

mechanical and optical parts


3 1 2 electron microscope

3.1.2 Electron microscope

Transmission electron microscope (TEM)

Scanning electron microscope (SEM)

SEM

TEM


Boys and girls welcome

TEM


Boys and girls welcome

Transmission electron microscope

(1)Features:★

① beam of electrons

② Electromagnetic lenses

③ Ultrathin (40-90 nm )

④ Heavy metal staining

(Uranyl acetate, Lead Citrate, lead nitrate)

(2)Dark areas of an electron micrograph are usually

called electron dense ★,

Light areas are called electronlucent★


Scanning electron microscope

Scanning electron microscope

Do not pass through the specimen,interact with surface of the specimen,

and produce reflected or emitted electrons→ → captured by a detector→ → transmit them to amplifiers and other devices→ → projected into a monitor→resulting in a pseudo-three-dimensional black-and-white image

SEM → →Pseudo-three-dimensional ,surface structures

TEM → →Plane, intra structures


Boys and girls welcome

3.2 Samples

3.2.1 Paraffin section(石蜡切片):

Classic and main sample

Steps of making paraffin section:For observing clearly

① Obtainthe sample

② Fix

③ Dehydrate, Clear

④ Embed

⑤ Section (5-10 um )

⑥ Stain(increase contrast)

⑦ Mount and label


Boys and girls welcome

3.2.2 0ther samples(self-study):

Frozen section

Ultrathin section

Smear

Stretched preparation

Ground section

Living cell


Boys and girls welcome

Smear

ground section

stretched preparation


3 treatments of samples

3 Treatments of samples

Treatments

AUSAS

3.1 H E staining:★

① The most commonly used

② H (hematoxylin),blue basic dye

make acid substance blue

③ E (eosin),red acidic dye

make basic substance red

④ Combination of hematoxylin and eosin

is called HE staining


3 1 1 terms relating to he staining

3.1.1 Terms relating to HE staining

a.★ Tissue components with an high affinity for basic dyes are termed basophilic

b.★ Tissue components with an high affinity for acid dyes are termed acidophilic

(Gr. Phileo means love)


3 1 2 rules of he staining

3.1.2 Rules of HE staining

A. Nucleus is generally stainedblue or purple

(why);

The more dark the nucleus stains, the more inactive the cell functions

B. Cytoplasm is generally stainedred(why);

RERs or ribosomes are stained blue in cytoplasm, which indicates cells actively synthesis proteins


3 2 histochemistry

3.2 Histochemistry

3.2.1 General histochemistry

1)Principles:★

target substances+A reagent→→insoluble colored or electron-dense compounds →→localization of target substances by means of light or electron microscopy

2)Localizing:

Ions

Polysaccharides & Oligosaccharides:PAS reaction

Lipids

Nucleic acids:Feulgen reaction

Proteins (enzymes)


3 2 2 immunohistochemistry

3.2.2 Immunohistochemistry

1)Princple:Specific affinity between antigen(target protein or peptide) and antibody

Lables:

Fluorescent compound,peroxidase,

gold particle


3 2 3 in situ hybridization

in situ hybridization

3.2.3 In situ hybridization

1)Probe: A known segment of single-stranded DNA or RNA that is complementary to the target nucleic acid. probe must be tagged with a lable.

2 ) Principle:Because of complementary→ probe+target nucleic acid→hybridizing→→detecting the target nucleic acid(DNA,RNA)


3 2 4 other treatments of samples self study

3.2.4 Other treatments of samples(self-study)

Special staining,

Vital staining

Heavy metal staining,

Autoradiography


4 about studying histology

4 About studying histology

1) preparation,lectures and review a lesson

2) systematic study:

Syetems, organs,tissues,cells, organelles

3) pay more attention to study method

4) asking and thinking

5) two dimentional and three dimentional

6) various sections of one structure

7) artifacts in tissue samples


Boys and girls welcome

Cell Membrane

Cytoplasm

Nucleus


Boys and girls welcome

obtain、fix

immerse

Dehydrate,clear

embed


Boys and girls welcome

section


Boys and girls welcome

stain

mount

lable


Boys and girls welcome

rules


Sliver staining

Sliver staining


Stretched preparation demonstrating macrophage

stretched preparation :demonstrating macrophage


Boys and girls welcome

Ground section of bone


Boys and girls welcome

This peripheral blood smear is stained with the Wright's stain

Blood smear, Giemsa stain.


Boys and girls welcome

PCNA阳性表达情况,免疫细胞化学染色,×400


Boys and girls welcome

免疫组织化学(荧光素标记)

毛细血管内皮细胞呈vWF阳性


Boys and girls welcome

OVER

TANAKS A LOT


  • Login