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Happy Early Thanksgiving!

Happy Early Thanksgiving!. Updates:. Quiz 10 grades are updated, new average: 76% Last quiz average: 78% Tools of the Light Trade assignment counts as one quiz grade! Scores are up Light Tools Findings grades will be posted by the end of the night

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Happy Early Thanksgiving!

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  1. Happy Early Thanksgiving!

  2. Updates: • Quiz 10 grades are updated, new average: 76% • Last quiz average: 78% • Tools of the Light Trade assignment counts as one quiz grade! Scores are up • Light Tools Findings grades will be posted by the end of the night • I’m still missing just a few GD peer evals…

  3. Photosynthesis and Electron Transport System

  4. Let’s think… • Electron transport… • REDOX reactions? • OIL RIG!

  5. Why we’re here: • Solve a question about the ‘invisible world’ through experimental design and execution • Design the experiment given tools you have and processes you’re familiar with • Derive the purpose and implementation of ‘controls’ for your experiment

  6. Where we’re going • Given: DCPIP takes electrons from Pq

  7. In groups of two… • What is DCPIP (Dichlorophenolindophenol)? • Does it share any characteristics with other molecules we’ve seen this semester? What? • What does it do? • How can you tell what it’s doing/what it’s done?

  8. Reduction of DCPIP:

  9. Claims: • DCPIP steals electrons • What effect do you predict this will have on photosynthesis/oxidation of H2O by Photosystem II? • An unknown herbicide blocks electron transport like a brick wall • What effects do you predict?

  10. Verify these claims! • How? Use the leaf disk assay we’ve been working with! • What conditions? • Key Question of the day: Where does the herbicide act? Before or after DCPIP?

  11. Will Photosynthesis happen in DCPIP? • How would you know? • Add 4ml DCPIP + 20mL buffer • ALSO, confirm an herbicide (Diuron) is a photosynthicide • Add 0.75mL + 20mL buffer* • Give your disks time to let the herbicide soak in • *Ignorethe change in volume adding these produce

  12. Evaluations • What did you learn? (Can be done individually while experiments progress) • What did you think of the semester (requires lab instructor to be finished & gone; but you can come back)

  13. Question of the day/week… X X WHERE does the herbicide work?

  14. Oh the thinks you can think! X DCPIP taking e- will do what to O2 prod? • DCPIP taking -e will do what to O2 prod? • How will -e taking affect DCPIP? • How can you tell if drug acts here? • Or here? X How will e- taking affect DCPIP? How can you tell if drug acts here? Or here?

  15. Oh the thinks you can think! • Two hypotheses? • What would your experiment look like? • What would it TELL you if the DCPIP… • Stays blue • Turns clear

  16. “Throw her into the pond!” • What tubes will you need? • Work it out now • Use the diagram

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