Device for converting elastin like polypeptide aggregate to soluble form
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Device for Converting Elastin-Like Polypeptide Aggregate to Soluble Form. Eric Lee, John Harrison, Albert Kwansa, Sasha Cai Lesher-Perez Client: Dr. Darin Furgeson Advising Professor: Dr. William Murphy. Overview of Presentation. Client research

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Device for converting elastin like polypeptide aggregate to soluble form

Device for Converting Elastin-Like Polypeptide Aggregate to Soluble Form

Eric Lee, John Harrison,

Albert Kwansa, Sasha Cai Lesher-Perez

Client: Dr. Darin Furgeson

Advising Professor: Dr. William Murphy


Overview of presentation
Overview of Presentation

  • Client research

  • Elastin-like polypeptides (ELP) introduction

  • Project design statement

  • Design specifications and assumptions

  • Proposed designs

  • Future Work

  • Answer questions


Client information
Client Information

  • Dr. Darin Furgeson from Pharmaceutical Sciences Department

  • Novel cancer treatment delivery system with ELP

    • Target specific cells

    • Non-viral

    • Lower toxicity


Background information on elp
Background Information on ELP

  • Synthetic protein

  • Repeating pentapeptide sequence

    (Val-Pro-Gly-Xaa-Gly)

  • Hydrophobic interaction

  • Thermal Responsive (transition temperature, Tt)

  • Non-viral vector for cancer treatment delivery


Current extraction process
Current Extraction Process

  • Transformation of plasmid into E. coli

  • Transcription and translation of ELP

  • Extraction of ELP from inclusion body

    (differential centrifugation)

  • ELP aggregation/separation from soluble DNA

  • Re-suspension


Project design statement

Design a device with temperature control, salt extraction, and particle reduction capabilities to enhance solubility of ELP aggregate while minimizing product loss.

Project Design Statement


Design specifications
Design Specifications and particle reduction capabilities to enhance solubility of ELP aggregate while minimizing product loss.

  • 75-80% yield of ELP

  • Maintenance below Tt

  • Durable material selection

  • Reduction of particle size

  • Portability


Assumptions for design

Mechanical stress and particle reduction capabilities to enhance solubility of ELP aggregate while minimizing product loss.

Various agitation methods have little degradative effects on ELP structure.

Heat Fluctuations

Changes to extreme

temperatures will not

damage ELP structural integrity.

Assumptions for Design


Freeze ELP in funnel and particle reduction capabilities to enhance solubility of ELP aggregate while minimizing product loss.

Initiate cone-shaped drill

Fall through non-stick tube

Descend into reservoir of cold PBS

Design #1

Device components

Operation

Cone-shaped drill

Funnel

Temperature control tank

Chilled PBS reservoir

with inlet


Design 1
Design #1 and particle reduction capabilities to enhance solubility of ELP aggregate while minimizing product loss.

  • Pros:

  • Frozen ELP: more manageable

  • Cons:

  • Electric power requirement due to use of motor.

  • Multiple parts requiring cooling.


Design 2
Design #2 and particle reduction capabilities to enhance solubility of ELP aggregate while minimizing product loss.

  • Operation

    • Place Frozen ELP on spinning blade

    • Force ELP through with plunger

    • Shavings fall in churning PBS bath

Blade

PBS Churner


Design 21
Design #2 and particle reduction capabilities to enhance solubility of ELP aggregate while minimizing product loss.

  • Pros

    • Requires less force to break down frozen ELP

    • Less manual intensive

    • Faster, cleaner

  • Cons

    • Very dynamic, many moving parts

    • Costly


Design 3
Design #3 and particle reduction capabilities to enhance solubility of ELP aggregate while minimizing product loss.

  • Operation

    • Coat screen with ELP aggregate

    • Submerge in cold PBS

    • Rotate at high speed/high torque

Motor

Shaft

PBS Tub

Non-stick Screen


Design 31
Design #3 and particle reduction capabilities to enhance solubility of ELP aggregate while minimizing product loss.

  • Pros

    • No frozen ELP

    • Simple, easy to operate

  • Cons

    • Material must meet unique design specifications

    • Force required to push ELP out may be much greater than anticipated


Future studies
Future Studies and particle reduction capabilities to enhance solubility of ELP aggregate while minimizing product loss.

  • Analyze/test feasibility of all three designs

    • Determine precise design specifications

    • Determine cost of manufacturing each design

    • Analyze complexity of each design

  • Refine designs for prototype


Questions? and particle reduction capabilities to enhance solubility of ELP aggregate while minimizing product loss.


More slides
More Slides and particle reduction capabilities to enhance solubility of ELP aggregate while minimizing product loss.

  • Chemotherapy

    • Non-specific

    • Highly toxic

    • Patients lose hair, feels sick, have no energy

  • ELP delivery system

    • ELP tagged medicine

    • Heat pad

    • Endocytosis

    • Lysosome


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