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(classical) genetics

(classical) genetics. causality. DNA. phenotype. ‘reverse’ genetics. T. C. The ‘gapped duplex’ method of in vitro mutagenesis. Gapped duplex method with phenotypic selection. Target : replace Trp by Phe Screening aid : Mnl I cleavage Mismatch primer : …GCCCT G GGCT TC GGTGGCA….

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(classical) genetics

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  1. (classical) genetics causality DNA phenotype ‘reverse’ genetics

  2. T C

  3. The ‘gapped duplex’ method of in vitro mutagenesis

  4. Gapped duplex method with phenotypic selection

  5. Target : replace Trp by Phe Screening aid : MnlI cleavage Mismatch primer : …GCCCTGGGCTTCGGTGGCA… Creating or removing a restriction recognition site as an aid in screening for site-specific mutants

  6. Codewoord tabel voor ‘reverse translation’ Leu = 1T2 Arg = 3G4 Ser = 567

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