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Joshua D. Shrout

Determining Surface Motility of Pseudomonas aeruginosa. Joshua D. Shrout. joshua.shrout@nd.edu. Motility affects biofilm formation. Pseudomonas aeruginosa. Gram negative rod-shaped bacterium Ubiquitous in nature

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Joshua D. Shrout

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  1. Determining Surface Motility of Pseudomonas aeruginosa Joshua D. Shrout joshua.shrout@nd.edu

  2. Motility affects biofilm formation

  3. Pseudomonas aeruginosa • Gram negative rod-shaped bacterium • Ubiquitous in nature • Opportunistic human pathogen (affects those with Cystic Fibrosis, skin burns, hospital-acquired infections, and contact lens wearers) • Model organism for study of bacterial group behavior

  4. Is surface motility variable?

  5. Cells do show distinct motility patterns Conrad, et al. (2011) Biophys.J.100: 1608-1616.

  6. P. aeruginosa has four surface motility patterns Conrad, et al. (2011) Biophys.J.100: 1608-1616.

  7. Cells “walk” vertically upright Gibiansky., et al. (2010) Science330: 197.

  8. Upright walking by daughter cells

  9. P. aeruginosa group behavior Type I: Rhamnolipid-independent swarming rhlAB-mutant swarming

  10. P. aeruginosa group behavior Type II: Rhamnolipid-dependent swarming Wild-type swarming

  11. Summary and Future work … • P. aeruginosa displays four distinct surface motility patterns, including upright “walking” that requires type IV pili. • Walking pattern suggests searching behavior. • Need to determine relevance of walking to group behavior on surfaces of interest (will start with skin assays in collaboration with Travers Lab). Skin assay (side view). Travers Laboratory IUSM

  12. Acknowledgements • Indiana Clinical and Translation Science Institute-Young Investigator Basic Science Award • Gerard Wong and Wong Lab, UCLA • Jeffery Travers and Travers Lab, IUSM • Robert Nerenberg and Shaun Lee, Notre Dame • Shrout Lab (and friends): Morgen Anyan, Andrew Courtney, Callan Driscoll, Cameron Harvey, Jessica Hewitt, Nachiket Kamatkar, Dominick Motto, Matthew Sarna, Geliang Song, Ann Staudt, Catherine Stecyk, and Lawrence Wolfe

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